The antiabortive effect of progesterone-induced blocking factor in mice is manifested by modulating NK activity

J. Szekeres-Barthó, G. Pár, Gy Dombay, Y. C. Smart, Z. Volgyi

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Abstract

Immunologic effects of progesterone are mediated by a protein named the progesterone-induced blocking factor (PIBF), which inhibits NK activity and displays an antiabortive effect in mice. Our previous data provide indirect evidence for the importance of PIBF in the maintenance of normal gestation. This study was aimed at investigating whether neutralization of endogenous PIBF production influences pregnancy outcome and if so, what are the mechanisms that participate in this process. Syngeneically pregnant Balb/c mice on Day 8.5 of pregnancy were injected ip with 0.3 mg/kg of RU 486 or with 0.5 mg of rabbit anti-PIBF IgG alone, or together with anti-NK monoclonal antibodies. Mice treated with the same amount of normal rabbit serum or untreated mice of similar gestational age were used as controls. On Day 10.5 the ratio of living and resorbed embryos and NK activity of the spleen cells were determined. In mice treated with anti-PIBF the ratio of resorbed fetuses was significantly higher than that in untreated controls. In RU 486-treated mice we also observed significantly increased resorption rate, which was associated with the inability of spleen cells to produce PIBF. Both anti-PIBF treatment and that with progesterone receptor blocker resulted in increased splenic NK activity. There was a positive relationship between NK activity and the rate of resorptions. All the above effects were corrected by simultaneous treatment with anti-NK or anti-NC (natural cytotoxic) antibodies. These data allow the conclusion that PIBF contributes to normal gestation in mice and that the effect of PIBF is manifested via blocking NK and/or NC activity.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalCellular Immunology
Volume177
Issue number2
DOIs
Publication statusPublished - May 1 1997

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Progesterone
Mifepristone
Pregnancy
Spleen
Rabbits
Mouse Pibf1 protein
blocking factor
Progesterone Receptors
Pregnancy Outcome
Gestational Age
Fetus
Embryonic Structures
Immunoglobulin G
Monoclonal Antibodies
Maintenance
Antibodies
Serum
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

The antiabortive effect of progesterone-induced blocking factor in mice is manifested by modulating NK activity. / Szekeres-Barthó, J.; Pár, G.; Dombay, Gy; Smart, Y. C.; Volgyi, Z.

In: Cellular Immunology, Vol. 177, No. 2, 01.05.1997, p. 194-199.

Research output: Contribution to journalArticle

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