The anti-apoptotic protein G1P3 is overexpressed in psoriasis and regulated by the non-coding RNA, PRINS

Krisztina Szegedi, Eniko Sonkoly, N. Nagy, István Balázs Németh, Z. Bata-Csörgő, L. Kemény, A. Dobozy, M. Széll

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Psoriasis Susceptibility-Related RNA Gene Induced by Stress (PRINS) is a non-coding RNA overexpressed in lesional and non-lesional psoriatic epidermis and induced by stress. Its function in healthy and psoriatic skin is still not known. Here, we report that PRINS regulates G1P3, a gene with anti-apoptotic effects in keratinocytes. siRNA-mediated inhibition of PRINS gene resulted in altered cell morphology and gene expression alterations, as demonstrated in a microarray experiment. One of the genes regulated by PRINS ncRNA was G1P3, an interferon-inducible gene with anti-apoptotic effects in cancer cells. Interestingly, we found that G1P3 was 400-fold upregulated in hyperproliferative lesional and ninefold upregulated in non-lesional psoriatic epidermis compared to healthy epidermis. In vitro, G1P3 protein levels were highest in proliferating keratinocytes and siRNA-mediated downregulation of G1P3 resulted in increased cell apoptosis. These data indicate that G1P3 inhibits spontaneous keratinocyte apoptosis and hence its high expression in psoriatic skin may contribute to the development of psoriatic lesions. We hypothesize that the deregulation of the PRINS ncRNA may contribute to psoriasis and results in decreased sensitivity to spontaneous keratinocyte apoptosis via the regulation of G1P3.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalExperimental Dermatology
Volume19
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Primed In Situ Labeling
Untranslated RNA
Apoptosis Regulatory Proteins
Psoriasis
Keratinocytes
Genes
Epidermis
Apoptosis
Small Interfering RNA
Skin
Deregulation
Microarrays
Gene expression
Interferons
Down-Regulation
Cells
RNA
Gene Expression
Neoplasms

Keywords

  • Apoptosis
  • G1P3
  • Non-coding RNA
  • Psoriasis

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

Cite this

The anti-apoptotic protein G1P3 is overexpressed in psoriasis and regulated by the non-coding RNA, PRINS. / Szegedi, Krisztina; Sonkoly, Eniko; Nagy, N.; Németh, István Balázs; Bata-Csörgő, Z.; Kemény, L.; Dobozy, A.; Széll, M.

In: Experimental Dermatology, Vol. 19, No. 3, 03.2010, p. 269-278.

Research output: Contribution to journalArticle

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