The anisin1 gene encodes a defensin-like protein and supports the fitness of Aspergillus nidulans

Andrea Eigentler, I. Pócsi, Florentine Marx

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the genome of Aspergillus nidulans, a defensin-like protein, Anisin1, was annotated with high homology to the mosquito defensin AaDefA1. So far, no studies exist on defensins from filamentous ascomycetes. Therefore, we characterized the anisin1 gene in A. nidulans and generated a deletion mutant, which suffered from a defect in mitospore development and produced less conidia at 42°C compared to the reference strain. In surface cultures of A. nidulans wild type, the anisin1 expression correlated with that of the central regulator for asexual development, brlA, and with the major scavanger of H 2O2 stress, catB, which is indicative for cell differentiation in developing fungi. Interestingly, brlA and anisin1 expressions were deregulated in a ΔsrrA strain that covers a central role in the histidine-to-aspartate (His-Asp) phosphorelay signaling pathway and shows impaired asexual development and H2O2 detoxification. In submers cultures of A. nidulans wild type and other mutants of the His-Asp phosphorelay signaling pathway, anisin1 was repressed, but derepressed in a ΔsrrA background, and anisin1 transcription was further increased in this mutant by H2O2 addition. We therefore conclude that the secreted protein Anisin1 contributes to the optimal development of A. nidulans and we further propose that it has a sensing/ signaling function for elevated H2O2 levels.

Original languageEnglish
Pages (from-to)427-437
Number of pages11
JournalArchives of Microbiology
Volume194
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Defensins
Aspergillus nidulans
Aspergillus
Genes
Histidine
Aspartic Acid
Detoxification
Proteins
Transcription
Fungi
Ascomycota
Fungal Spores
Defects
Culicidae
Cell Differentiation
Genome

Keywords

  • Asexual development
  • Aspergillus nidulans
  • Defensin
  • Oxidative stress

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Biochemistry
  • Genetics

Cite this

The anisin1 gene encodes a defensin-like protein and supports the fitness of Aspergillus nidulans. / Eigentler, Andrea; Pócsi, I.; Marx, Florentine.

In: Archives of Microbiology, Vol. 194, No. 6, 06.2012, p. 427-437.

Research output: Contribution to journalArticle

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