The analysis of phenylthiocarbamyl amino acids in the presence of sodium chloride

Mohammed Khalifa, I. Molnár-Perl

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This article reports on the impact of sodium chloride (NaCl) on hydrolysis and derivatization (using phenylisothiocyanate) of amino acids obtained in hydrolysates from standard proteins and from matrices containing high levels of proteins. The presence of NaCl (in both hydrolysis and derivatization) at NaCl/protein m/m ratios as high as approximately 100/1 resulted in amino acid recoveries of almost 100%, with some exceptions. Thus, salt-elimination procedures, such as ion exchange and solid-phase extraction, which often lead to recovery losses, can be omitted.

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalLC-GC Europe
Volume9
Issue number3
Publication statusPublished - 1996

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Sodium Chloride
Amino Acids
Hydrolysis
Recovery
Proteins
Ion exchange
Salts
phenylisothiocyanate

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

The analysis of phenylthiocarbamyl amino acids in the presence of sodium chloride. / Khalifa, Mohammed; Molnár-Perl, I.

In: LC-GC Europe, Vol. 9, No. 3, 1996, p. 143-146.

Research output: Contribution to journalArticle

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