The analysis of MHC class II DRB1, -DQB1, -DQA1 genes in ragweed sensitive asthmatic children

Research output: Contribution to journalArticle

Abstract

Background: Certain alleles within the class II human leukocyte antigen (HLA) gene-complex are associated with sensitivity caused by allergens. The authors have evaluated the distribution of HLA-DRB1, -DQA1, -DQB1 alleles in asthmatic children with bronchial asthma caused by Ambrosia artemisiifolia (Amb a V). Patients and methods: 11 patients (aged: 4-14 year, boy:girl = 10:1) have participated in this study. The selection was randomly done according to clinical signs, the positive skin prick test and/or the increased fetal IgE serum level. Data were compared to control group consisting of 50 healthy blood donor adults. Polymerase chain reaction (PCR) and allele specific hybridizations were carried out for DRB1 by using Amplicor® PCR Diagnostic Kit (Hoffmann-La Roche) followed by Ota's PCR-RFLP method to subtype the DR2 alleles, for DQA1 by using Ota's PCR-RFLP method for DQB1 using Mercier's PCR-RFLP method. Statistical analysis was performed by using chi square test with Yates correction. Results and conclusions: HLA-DQB1(*)0201, (*)0202 (p = 0,005) and (*)0602 (p = 0,05) alleles were significantly increasedwhile HLA-DQB1(*)0301 (p <0,05) allele was significantly decreased in the examined children.

Original languageEnglish
Pages (from-to)493-495
Number of pages3
JournalPeriodicum Biologorum
Volume98
Issue number4
Publication statusPublished - 1996

Fingerprint

Ambrosia
Polymerase chain reaction
HLA Antigens
Genes
Alleles
alleles
polymerase chain reaction
Polymerase Chain Reaction
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
genes
HLA-D Antigens
Ambrosia artemisiifolia
analytical kits
skin tests
Allergens
Immunoglobulin E
asthma
Chi-Square Distribution
allergens

Keywords

  • Asthmatic children
  • MHC class II
  • Ragweed

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

@article{264b3c43fc164a609ca7c16a412bb0e0,
title = "The analysis of MHC class II DRB1, -DQB1, -DQA1 genes in ragweed sensitive asthmatic children",
abstract = "Background: Certain alleles within the class II human leukocyte antigen (HLA) gene-complex are associated with sensitivity caused by allergens. The authors have evaluated the distribution of HLA-DRB1, -DQA1, -DQB1 alleles in asthmatic children with bronchial asthma caused by Ambrosia artemisiifolia (Amb a V). Patients and methods: 11 patients (aged: 4-14 year, boy:girl = 10:1) have participated in this study. The selection was randomly done according to clinical signs, the positive skin prick test and/or the increased fetal IgE serum level. Data were compared to control group consisting of 50 healthy blood donor adults. Polymerase chain reaction (PCR) and allele specific hybridizations were carried out for DRB1 by using Amplicor{\circledR} PCR Diagnostic Kit (Hoffmann-La Roche) followed by Ota's PCR-RFLP method to subtype the DR2 alleles, for DQA1 by using Ota's PCR-RFLP method for DQB1 using Mercier's PCR-RFLP method. Statistical analysis was performed by using chi square test with Yates correction. Results and conclusions: HLA-DQB1(*)0201, (*)0202 (p = 0,005) and (*)0602 (p = 0,05) alleles were significantly increasedwhile HLA-DQB1(*)0301 (p <0,05) allele was significantly decreased in the examined children.",
keywords = "Asthmatic children, MHC class II, Ragweed",
author = "O. Bede and K. Gyurkovits and E. Endreffy and S. Pint{\'e}r",
year = "1996",
language = "English",
volume = "98",
pages = "493--495",
journal = "Periodicum Biologorum",
issn = "0031-5362",
publisher = "Croatian Society of Natural Sciences",
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}

TY - JOUR

T1 - The analysis of MHC class II DRB1, -DQB1, -DQA1 genes in ragweed sensitive asthmatic children

AU - Bede, O.

AU - Gyurkovits, K.

AU - Endreffy, E.

AU - Pintér, S.

PY - 1996

Y1 - 1996

N2 - Background: Certain alleles within the class II human leukocyte antigen (HLA) gene-complex are associated with sensitivity caused by allergens. The authors have evaluated the distribution of HLA-DRB1, -DQA1, -DQB1 alleles in asthmatic children with bronchial asthma caused by Ambrosia artemisiifolia (Amb a V). Patients and methods: 11 patients (aged: 4-14 year, boy:girl = 10:1) have participated in this study. The selection was randomly done according to clinical signs, the positive skin prick test and/or the increased fetal IgE serum level. Data were compared to control group consisting of 50 healthy blood donor adults. Polymerase chain reaction (PCR) and allele specific hybridizations were carried out for DRB1 by using Amplicor® PCR Diagnostic Kit (Hoffmann-La Roche) followed by Ota's PCR-RFLP method to subtype the DR2 alleles, for DQA1 by using Ota's PCR-RFLP method for DQB1 using Mercier's PCR-RFLP method. Statistical analysis was performed by using chi square test with Yates correction. Results and conclusions: HLA-DQB1(*)0201, (*)0202 (p = 0,005) and (*)0602 (p = 0,05) alleles were significantly increasedwhile HLA-DQB1(*)0301 (p <0,05) allele was significantly decreased in the examined children.

AB - Background: Certain alleles within the class II human leukocyte antigen (HLA) gene-complex are associated with sensitivity caused by allergens. The authors have evaluated the distribution of HLA-DRB1, -DQA1, -DQB1 alleles in asthmatic children with bronchial asthma caused by Ambrosia artemisiifolia (Amb a V). Patients and methods: 11 patients (aged: 4-14 year, boy:girl = 10:1) have participated in this study. The selection was randomly done according to clinical signs, the positive skin prick test and/or the increased fetal IgE serum level. Data were compared to control group consisting of 50 healthy blood donor adults. Polymerase chain reaction (PCR) and allele specific hybridizations were carried out for DRB1 by using Amplicor® PCR Diagnostic Kit (Hoffmann-La Roche) followed by Ota's PCR-RFLP method to subtype the DR2 alleles, for DQA1 by using Ota's PCR-RFLP method for DQB1 using Mercier's PCR-RFLP method. Statistical analysis was performed by using chi square test with Yates correction. Results and conclusions: HLA-DQB1(*)0201, (*)0202 (p = 0,005) and (*)0602 (p = 0,05) alleles were significantly increasedwhile HLA-DQB1(*)0301 (p <0,05) allele was significantly decreased in the examined children.

KW - Asthmatic children

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