The aluminium(III)-sialic acid interaction: A potential role in aluminium-induced cellular membrane degeneration

V. B. Di Marco, A. Károly-Lakatos, A. Venzo, R. Bertani, R. Seraglia, T. Kiss

Research output: Contribution to journalArticle

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Abstract

The coordination between Al(III) and sialic acid (N-acetylneuraminic acid, HL, pKa = 2.58 ± 0.01) was studied by potentiometric titrations at 25 °C in aqueous 0.2 M KCl, by 1H NMR, and by electrospray ionization mass spectrometry (ESI-MS). The potentiometric measurements gave the following aluminium complex stoichiometries and stability constants: log β (AlLH- 1+) = - 1.28 ± 0.01, log β(AlLH-2) = -6.34 ± 0.02, and log β(AlL2H-1) = -1.14 ± 0.04. The 1H NMR spectra yielded structural information on species AlLH- 1+. The ESI-MS data confirmed the metal-ligand stoichiometry of the complexes. The metal-ligand speciation at micromolar Al(III) concentrations (i.e., under in vivo conditions) at physiological pH values reveals that considerable amount of Al(III) is complexed. This suggests that the toxic effect of Al(III) towards cellular membranes might be due to its coordination by protein-bound sialic acid.

Original languageEnglish
Pages (from-to)4227-4234
Number of pages8
JournalInorganica Chimica Acta
Volume359
Issue number13
DOIs
Publication statusPublished - Oct 1 2006

Fingerprint

degeneration
N-Acetylneuraminic Acid
Aluminum
Electrospray ionization
Electrospray Ionization Mass Spectrometry
membranes
Membranes
aluminum
Stoichiometry
acids
Mass spectrometry
Acids
stoichiometry
mass spectroscopy
Metals
Ligands
Nuclear magnetic resonance
ionization
nuclear magnetic resonance
ligands

Keywords

  • Al(III)
  • Aluminium toxicity
  • ESI-MS
  • NMR
  • Potentiometry
  • Sialic acid

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry
  • Physical and Theoretical Chemistry
  • Materials Chemistry

Cite this

The aluminium(III)-sialic acid interaction : A potential role in aluminium-induced cellular membrane degeneration. / Di Marco, V. B.; Károly-Lakatos, A.; Venzo, A.; Bertani, R.; Seraglia, R.; Kiss, T.

In: Inorganica Chimica Acta, Vol. 359, No. 13, 01.10.2006, p. 4227-4234.

Research output: Contribution to journalArticle

Di Marco, V. B. ; Károly-Lakatos, A. ; Venzo, A. ; Bertani, R. ; Seraglia, R. ; Kiss, T. / The aluminium(III)-sialic acid interaction : A potential role in aluminium-induced cellular membrane degeneration. In: Inorganica Chimica Acta. 2006 ; Vol. 359, No. 13. pp. 4227-4234.
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