The adenosine A2A receptor agonist CGS 21680 fails to ameliorate the course of dextran sulphate-induced colitis in mice

Z. Selmeczy, B. Csóka, P. Pacher, E. Vízi, G. Haskó

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: In this study we investigated the effect of CGS 21680 (2-p-(2-Carboxyethyl)phenethylamino-5-N-ethylcarboxamidoadenosine hydrochloride), an adenosine A2A receptor agonist, in a model of dextran sulphate sodium (DSS)-induced colitis. Methods: NMRI mice were fed 5 % (w/v) DSS, and were treated intraperitoneally with 0.5 mg/kg CGS 21680 or vehicle for 10 days. Changes of bodyweight, colon length, the incidence of rectal bleeding, levels of macrophage inflammatory protein (MIP)-1α, MIP-2, interferon γ, interleukin (IL)-1β, IL-12 and tumour necrosis factor-α from homogenates of colon biopsies, and the release of [ 3H]acetylcholine (ACh) from longitudinal muscle strip were determined. Results: DSS significantly decreased bodyweight, colon length, and it increased the incidence of rectal bleeding and levels of MIP-1α, MIP-2 and IL-1β compared to DSS-untreated animals. CGS 21680 had no effect on these changes. No change could be observed in release of ACh in DSS-induced colitis with or without CGS 21680. Conclusion: In summary, CGS 21680 is ineffective in ameliorating DSS-induced colitis in mice.

Original languageEnglish
Pages (from-to)204-209
Number of pages6
JournalInflammation Research
Volume56
Issue number5
DOIs
Publication statusPublished - May 2007

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Adenosine A2 Receptor Agonists
Dextran Sulfate
Colitis
Chemokine CXCL2
Macrophage Inflammatory Proteins
Colon
Interleukin-1
Acetylcholine
Hemorrhage
Biopsy
Incidence
Interleukin-12
Interferons
Muscle
2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine
Animals
Tumor Necrosis Factor-alpha
Muscles

Keywords

  • Adenosine A receptor
  • CGS 21680
  • Colitis
  • Dextran sulphate

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

The adenosine A2A receptor agonist CGS 21680 fails to ameliorate the course of dextran sulphate-induced colitis in mice. / Selmeczy, Z.; Csóka, B.; Pacher, P.; Vízi, E.; Haskó, G.

In: Inflammation Research, Vol. 56, No. 5, 05.2007, p. 204-209.

Research output: Contribution to journalArticle

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