The action of a synthetic derivative of Met5-enkephalin-Arg 6-Phe7 on behavioral and endocrine responses

Krisztina Csabafi, Miklós Jászberényi, Zsolt Bagosi, Géza Tóth, Mária Wollemann, Gyula Telegdy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The neuroendocrine and behavioral effects of Tyr-d-Ala-Gly-Phe-d-Nle-Arg- Phe (DADN), a more stable derivative of the endogenous opiate Met-enkephalin related peptide Met5-enkephalin-Arg6-Phe7 were investigated in mice. The behavioral experiments consisted of monitoring the horizontal (square crossing) and vertical (rearing) locomotion in the open field system. To evaluate the effect of the heptapeptide on the hypothalamo- pituitary-adrenal (HPA) axis, the plasma corticosterone level was measured. DADN induced dose-dependent increases in locomotion and rearing 30 min after intracerebroventricular injection and also elicited marked activation of the hormonal stress response. To elucidate the receptors involved in the mediation of these actions, animals were pretreated with the nonselective opioid antagonist naloxone, the selective κ-receptor antagonist nor-binaltorphimine or the μ1-receptor blocker naloxonazine. Both the HPA activation and the behavioral responses were diminished by the preadministration of naloxone. Nor-binaltorphimine did not display a significant effect, while naloxonazine completely abolished the hyperactivity and the corticosterone elevation elicited by the analog. These findings suggest that μ-receptors predominate in the mediation of the neuroendocrine actions of DADN, while κ-receptors do not play a significant role.

Original languageEnglish
Pages (from-to)1656-1660
Number of pages5
JournalPeptides
Volume32
Issue number8
DOIs
Publication statusPublished - Aug 2011

Keywords

  • Hypothalamic-pituitary-adrenal axis
  • Open field
  • Opioid receptors

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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