The action of a synthetic derivative of Met 5-enkephalin-Arg 6-Phe 7 on behavioral and endocrine responses

Krisztina Csabafi, M. Jászberényi, Zsolt Bagosi, Géza Tóth, Mária Wollemann, G. Telegdy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The neuroendocrine and behavioral effects of Tyr-d-Ala-Gly-Phe-d-Nle-Arg- Phe (DADN), a more stable derivative of the endogenous opiate Met-enkephalin related peptide Met 5-enkephalin-Arg 6-Phe 7 were investigated in mice. The behavioral experiments consisted of monitoring the horizontal (square crossing) and vertical (rearing) locomotion in the open field system. To evaluate the effect of the heptapeptide on the hypothalamo- pituitary-adrenal (HPA) axis, the plasma corticosterone level was measured. DADN induced dose-dependent increases in locomotion and rearing 30 min after intracerebroventricular injection and also elicited marked activation of the hormonal stress response. To elucidate the receptors involved in the mediation of these actions, animals were pretreated with the nonselective opioid antagonist naloxone, the selective κ-receptor antagonist nor-binaltorphimine or the μ 1-receptor blocker naloxonazine. Both the HPA activation and the behavioral responses were diminished by the preadministration of naloxone. Nor-binaltorphimine did not display a significant effect, while naloxonazine completely abolished the hyperactivity and the corticosterone elevation elicited by the analog. These findings suggest that μ-receptors predominate in the mediation of the neuroendocrine actions of DADN, while κ-receptors do not play a significant role.

Original languageEnglish
Pages (from-to)1656-1660
Number of pages5
JournalPeptides
Volume32
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Methionine Enkephalin
Locomotion
Naloxone
Corticosterone
Chemical activation
Derivatives
Opioid Peptides
Narcotic Antagonists
Animals
Plasmas
Peptides
Injections
Monitoring
Experiments
naloxonazine
norbinaltorphimine
naloxone receptor

Keywords

  • Hypothalamic-pituitary-adrenal axis
  • Met -enkephalin-Arg -Phe
  • Open field
  • Opioid receptors

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

The action of a synthetic derivative of Met 5-enkephalin-Arg 6-Phe 7 on behavioral and endocrine responses. / Csabafi, Krisztina; Jászberényi, M.; Bagosi, Zsolt; Tóth, Géza; Wollemann, Mária; Telegdy, G.

In: Peptides, Vol. 32, No. 8, 08.2011, p. 1656-1660.

Research output: Contribution to journalArticle

Csabafi, Krisztina ; Jászberényi, M. ; Bagosi, Zsolt ; Tóth, Géza ; Wollemann, Mária ; Telegdy, G. / The action of a synthetic derivative of Met 5-enkephalin-Arg 6-Phe 7 on behavioral and endocrine responses. In: Peptides. 2011 ; Vol. 32, No. 8. pp. 1656-1660.
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