The action in vivo of glycine betaine in enhancement of tolerance of Synechococcus sp. strain PCC 7942 to low temperature

Patcharaporn Deshnium, Z. Gombos, Yoshitaka Nishiyama, Norio Murata

Research output: Contribution to journalArticle

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Abstract

The cyanobacterium Synechococcus sp. strain PCC 7942 was transformed with the codA gene for choline oxidase from Arthrobacter globiformis under the control of a constitutive promoter. This transformation allowed the cyanobacterial cells to accumulate glycine betaine at 60 to 80 mM in the cytoplasm. The transformed cells could grow at 20°C, the temperature at which the growth of control cells was markedly suppressed. Photosynthesis of the transformed cells at 20°C was more tolerant to light than that of the control cells. This was caused by the enhanced ability of the photosynthetic machinery in the transformed cells to recover from low-temperature photoinhibition. In darkness, photosynthesis of the transformed cells was more tolerant to low temperature such as 0 to 10°C than that of the control cells. In parallel with the improvement in the ability of the transformed cells to tolerate low temperature, the lipid phase transition of plasma membranes from the liquid-crystalline state to the gel state shifted toward lower temperatures, although the level of unsaturation of the membrane lipids was unaffected by the transformation. These findings suggest that glycine betaine enhances the tolerance of photosynthesis to low temperature.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalJournal of Bacteriology
Volume179
Issue number2
Publication statusPublished - 1997

Fingerprint

Synechococcus
Betaine
Temperature
Photosynthesis
choline oxidase
Arthrobacter
Darkness
Phase Transition
Cyanobacteria
Membrane Lipids
Cytoplasm
Gels
Cell Membrane
Lipids
Light

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The action in vivo of glycine betaine in enhancement of tolerance of Synechococcus sp. strain PCC 7942 to low temperature. / Deshnium, Patcharaporn; Gombos, Z.; Nishiyama, Yoshitaka; Murata, Norio.

In: Journal of Bacteriology, Vol. 179, No. 2, 1997, p. 339-344.

Research output: Contribution to journalArticle

Deshnium, Patcharaporn ; Gombos, Z. ; Nishiyama, Yoshitaka ; Murata, Norio. / The action in vivo of glycine betaine in enhancement of tolerance of Synechococcus sp. strain PCC 7942 to low temperature. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 2. pp. 339-344.
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