The abc1 - /coq8 - respiratory-deficient mutant of Schizosaccharomyces pombe suffers from glutathione underproduction and hyperaccumulates Cd 2+

Zoltan Gazdag, Stefan Fujs, Balázs Kőszegi, Nikoletta Kálmán, Gábor Papp, Tamás Emri, Joseph Belágyi, István Pócsi, Peter Raspor, Miklós Pesti

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12 Citations (Scopus)

Abstract

The abc1 - /coq8 - gene deletion respiratory-deficient mutant NBp17 of fission yeast Schizosaccharomyces pombe displayed a phenotypic fermentation pattern with enhanced production of glycerol and acetate, and also possessed oxidative stress-sensitive phenotypes to H 2 O 2 , menadione, tBuOOH, Cd 2+ , and chromate in comparison with its parental respiratory-competent strain HNT. As a consequence of internal stress-inducing mutation, adaptation processes to restore the redox homeostasis of mutant NBp17 cells were detected in minimal glucose medium. Mutant NBp17 produced significantly increased amounts of O 2 •- and H 2 O 2 as a result of the decreased internal glutathione concentration and the only slightly increased glutathione reductase activity. The Cr(VI) reduction capacity and hence the OH production ability were decreased. The mutant cells demonstrated increased specific activities of superoxide dismutases and glutathione reductase (but not catalase) to detoxify at least partially the overproduction of reactive oxygen species. All these features may be explained by the decreased redox capacity of the mutant cells. Most notably, mutant NBp17 hyperaccumulated yellow CdS.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalFolia Microbiologica
Volume56
Issue number4
DOIs
Publication statusPublished - Jul 2011

ASJC Scopus subject areas

  • Microbiology

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