The AAA+ ATPases and HflB/FtsH proteases of 'Candidatus Phytoplasma mali': Phylogenetic diversity, membrane topology, and relationship to strain virulence

Erich Seemüller, S. Süle, Michael Kube, Wilhelm Jelkmann, Bernd Schneider

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previous examination revealed a correlation of phytopathogenic data of 'Candidatus Phytoplasma mali' strains and the DNA sequence variability of a type ATP00464 hflB gene fragment. To further investigate such a relationship, all distinct genes previously annotated as hflB in the genome of 'Ca. P. mali' strain AT were fully sequenced and analyzed from a number of representative mild, moderate, and severe strains. The re-annotation indicated that the sequences encode six AAA+ ATPases and six HflB proteases. Each of the nine distinct deduced AAA+ proteins that were examined formed a coherent phylogenetic cluster. However, within these groups, sequences of three ATPases and three proteases from mild and severe strains clustered distantly, according to their virulence. This grouping was supported by an association with virulence-related amino acid substitutions. Another finding was that full-length genes from ATPase AP11 could only be identified in mild and moderate strains. Prediction of the membrane topology indicated that the long ATPase- and protease-carrying C-terminal tails of approximately half of the AAA+ proteins are extracellular, putatively facing the environment of the sieve tubes. Thus, they may be involved in pathogen-host interactions and may compromise phloem function, a major effect of phytoplasma infection. All full-length genes examined appear transcriptionally active and all deduced peptides show the key positions indicative for protein function.

Original languageEnglish
Pages (from-to)367-376
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume26
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Phytoplasma
Mali
topology
adenosinetriphosphatase
Virulence
Adenosine Triphosphatases
Peptide Hydrolases
virulence
proteinases
Membranes
phylogeny
Genes
phytoplasmal diseases
Phloem
Host-Pathogen Interactions
genes
Proteins
Amino Acid Substitution
sieve tubes
host-pathogen relationships

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

The AAA+ ATPases and HflB/FtsH proteases of 'Candidatus Phytoplasma mali' : Phylogenetic diversity, membrane topology, and relationship to strain virulence. / Seemüller, Erich; Süle, S.; Kube, Michael; Jelkmann, Wilhelm; Schneider, Bernd.

In: Molecular Plant-Microbe Interactions, Vol. 26, No. 3, 03.2013, p. 367-376.

Research output: Contribution to journalArticle

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