The 10 S BC-1 ribonucleoprotein particle contains identifier sequence- binding proteins that interact with an array of GCAAG/CTTGC motifs between split promoter sequences for RNA polymerase III

S. Kobayashi, N. Higashi, K. Suzuki, S. Goto, K. Yumoto, K. Anzai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

BC-1 RNA is a brain-specific small RNA transcript of identifier sequences present in the somas and dendrites of neurons. We recently reported that the RNA is complexed with a protein(s) to form a 10 S ribonucleoprotein particle (Kobayashi, S., Goto, S., and Anzai, K. (1991) J. Biol. Chem. 266, 4726- 4730). We demonstrate here that this 10 S BC-1 ribonucleoprotein particle contains a DNA-binding protein(s) (Bp-1 protein) capable of interacting with a region between split promoter sequences for RNA polymerase III within the identifier sequences. The region has short inverted repeats: a perfect octanucleotide repeat (GCGCTTGCCTAGCAAGCGC) and an imperfect heptanucleotide repeat (GCCTAGCAAGCGCAAGGC), each of which contains a GCAAG/CTTGC motif. We also demonstrate that the binding of this protein either to the array of pentamer motifs or to BC-1 RNA is mutually exclusive. The molecular masses of photo-cross-linking adducts of Bp-1 protein to a 32P-labeled GCAAG/CTTGC motif-specific probe were estimated to be about 31 and 36 kDa, indicating that two species of Bp-1 proteins may be present in the brain.

Original languageEnglish
Pages (from-to)18291-18297
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number26
Publication statusPublished - Jan 1 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The 10 S BC-1 ribonucleoprotein particle contains identifier sequence- binding proteins that interact with an array of GCAAG/CTTGC motifs between split promoter sequences for RNA polymerase III'. Together they form a unique fingerprint.

  • Cite this