Th1/Th2 imbalance, measured by circulating and intracytoplasmic inflammatory cytokines - Immunological alterations in acute coronary syndrome and stable coronary artery disease

P. Szodoray, O. Timar, K. Veres, H. Dér, E. Szomjak, G. Lakos, M. Aleksza, B. Nakken, G. Szegedi, P. Soltész

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

To describe how peripheral immune-parameters reflect the inflammatory alterations of the atherosclerotic plaques in coronary atherosclerosis. We measured general inflammatory markers C-reactive protein (CRP) and granulocyte activity, lymphocyte subpopulations and their state of activation, evaluated circulating Th1/Th2-type cytokines, and specific intracytoplasmic cytokines. We investigated the association of immune-parameters with disease outcome and mortality. Thirty-three patients with acute coronary syndrome (ACS), 62 with stable coronary artery disease (CAD) and 58 healthy controls were studied. Peripheral blood lymphocyte subgroups were quantified by flow cytometry, soluble cytokines and autoantibodies were assessed using enzyme-linked immunosorbent assay (ELISA), while intracellular cytokine levels were measured by flow cytometry after intracellular staining. We found elevated levels of CRP and granulocyte activity in ACS versus CAD (P <0.001, P = 0.017, respectively). Natural killer (NK) cell percentages were elevated, while percentage of T cells to the total lymphocyte count was slightly decreased in ACS compared to controls (P <0.0001, P = 0.012, respectively). Both forms of coronary atherosclerosis showed significantly higher percentages of activated T cells than controls when stained for the activation markers HLA-DR3 and CD69+ (ACS: P <0.0001, P = 0.002, CAD: P <0.0001, P = 0.018, respectively). IL-1, IL-4 and IL-10 proved significantly higher in ACS versus controls (P = 0.036, P = 0.01, P <0.0001 respectively). Th1 to Th2 ratio shifted towards a Th1 dominance in both diseases. Both general proinflammatory markers and activated T cells signify CAD. The orchestrated proinflammatory cascade eventually leads to the development of the disease.

Original languageEnglish
Pages (from-to)336-344
Number of pages9
JournalScandinavian Journal of Immunology
Volume64
Issue number3
DOIs
Publication statusPublished - Sep 2006

Fingerprint

Acute Coronary Syndrome
Coronary Artery Disease
Cytokines
T-Lymphocytes
Granulocytes
C-Reactive Protein
Flow Cytometry
HLA-DR3 Antigen
Lymphocyte Subsets
Lymphocyte Count
Atherosclerotic Plaques
Interleukin-1
Natural Killer Cells
Interleukin-4
Interleukin-10
Autoantibodies
Enzyme-Linked Immunosorbent Assay
Lymphocytes
Staining and Labeling
Mortality

ASJC Scopus subject areas

  • Immunology

Cite this

@article{07e3e13d2a0043ce94b0e9ffb2e4c637,
title = "Th1/Th2 imbalance, measured by circulating and intracytoplasmic inflammatory cytokines - Immunological alterations in acute coronary syndrome and stable coronary artery disease",
abstract = "To describe how peripheral immune-parameters reflect the inflammatory alterations of the atherosclerotic plaques in coronary atherosclerosis. We measured general inflammatory markers C-reactive protein (CRP) and granulocyte activity, lymphocyte subpopulations and their state of activation, evaluated circulating Th1/Th2-type cytokines, and specific intracytoplasmic cytokines. We investigated the association of immune-parameters with disease outcome and mortality. Thirty-three patients with acute coronary syndrome (ACS), 62 with stable coronary artery disease (CAD) and 58 healthy controls were studied. Peripheral blood lymphocyte subgroups were quantified by flow cytometry, soluble cytokines and autoantibodies were assessed using enzyme-linked immunosorbent assay (ELISA), while intracellular cytokine levels were measured by flow cytometry after intracellular staining. We found elevated levels of CRP and granulocyte activity in ACS versus CAD (P <0.001, P = 0.017, respectively). Natural killer (NK) cell percentages were elevated, while percentage of T cells to the total lymphocyte count was slightly decreased in ACS compared to controls (P <0.0001, P = 0.012, respectively). Both forms of coronary atherosclerosis showed significantly higher percentages of activated T cells than controls when stained for the activation markers HLA-DR3 and CD69+ (ACS: P <0.0001, P = 0.002, CAD: P <0.0001, P = 0.018, respectively). IL-1, IL-4 and IL-10 proved significantly higher in ACS versus controls (P = 0.036, P = 0.01, P <0.0001 respectively). Th1 to Th2 ratio shifted towards a Th1 dominance in both diseases. Both general proinflammatory markers and activated T cells signify CAD. The orchestrated proinflammatory cascade eventually leads to the development of the disease.",
author = "P. Szodoray and O. Timar and K. Veres and H. D{\'e}r and E. Szomjak and G. Lakos and M. Aleksza and B. Nakken and G. Szegedi and P. Solt{\'e}sz",
year = "2006",
month = "9",
doi = "10.1111/j.1365-3083.2006.01816.x",
language = "English",
volume = "64",
pages = "336--344",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Th1/Th2 imbalance, measured by circulating and intracytoplasmic inflammatory cytokines - Immunological alterations in acute coronary syndrome and stable coronary artery disease

AU - Szodoray, P.

AU - Timar, O.

AU - Veres, K.

AU - Dér, H.

AU - Szomjak, E.

AU - Lakos, G.

AU - Aleksza, M.

AU - Nakken, B.

AU - Szegedi, G.

AU - Soltész, P.

PY - 2006/9

Y1 - 2006/9

N2 - To describe how peripheral immune-parameters reflect the inflammatory alterations of the atherosclerotic plaques in coronary atherosclerosis. We measured general inflammatory markers C-reactive protein (CRP) and granulocyte activity, lymphocyte subpopulations and their state of activation, evaluated circulating Th1/Th2-type cytokines, and specific intracytoplasmic cytokines. We investigated the association of immune-parameters with disease outcome and mortality. Thirty-three patients with acute coronary syndrome (ACS), 62 with stable coronary artery disease (CAD) and 58 healthy controls were studied. Peripheral blood lymphocyte subgroups were quantified by flow cytometry, soluble cytokines and autoantibodies were assessed using enzyme-linked immunosorbent assay (ELISA), while intracellular cytokine levels were measured by flow cytometry after intracellular staining. We found elevated levels of CRP and granulocyte activity in ACS versus CAD (P <0.001, P = 0.017, respectively). Natural killer (NK) cell percentages were elevated, while percentage of T cells to the total lymphocyte count was slightly decreased in ACS compared to controls (P <0.0001, P = 0.012, respectively). Both forms of coronary atherosclerosis showed significantly higher percentages of activated T cells than controls when stained for the activation markers HLA-DR3 and CD69+ (ACS: P <0.0001, P = 0.002, CAD: P <0.0001, P = 0.018, respectively). IL-1, IL-4 and IL-10 proved significantly higher in ACS versus controls (P = 0.036, P = 0.01, P <0.0001 respectively). Th1 to Th2 ratio shifted towards a Th1 dominance in both diseases. Both general proinflammatory markers and activated T cells signify CAD. The orchestrated proinflammatory cascade eventually leads to the development of the disease.

AB - To describe how peripheral immune-parameters reflect the inflammatory alterations of the atherosclerotic plaques in coronary atherosclerosis. We measured general inflammatory markers C-reactive protein (CRP) and granulocyte activity, lymphocyte subpopulations and their state of activation, evaluated circulating Th1/Th2-type cytokines, and specific intracytoplasmic cytokines. We investigated the association of immune-parameters with disease outcome and mortality. Thirty-three patients with acute coronary syndrome (ACS), 62 with stable coronary artery disease (CAD) and 58 healthy controls were studied. Peripheral blood lymphocyte subgroups were quantified by flow cytometry, soluble cytokines and autoantibodies were assessed using enzyme-linked immunosorbent assay (ELISA), while intracellular cytokine levels were measured by flow cytometry after intracellular staining. We found elevated levels of CRP and granulocyte activity in ACS versus CAD (P <0.001, P = 0.017, respectively). Natural killer (NK) cell percentages were elevated, while percentage of T cells to the total lymphocyte count was slightly decreased in ACS compared to controls (P <0.0001, P = 0.012, respectively). Both forms of coronary atherosclerosis showed significantly higher percentages of activated T cells than controls when stained for the activation markers HLA-DR3 and CD69+ (ACS: P <0.0001, P = 0.002, CAD: P <0.0001, P = 0.018, respectively). IL-1, IL-4 and IL-10 proved significantly higher in ACS versus controls (P = 0.036, P = 0.01, P <0.0001 respectively). Th1 to Th2 ratio shifted towards a Th1 dominance in both diseases. Both general proinflammatory markers and activated T cells signify CAD. The orchestrated proinflammatory cascade eventually leads to the development of the disease.

UR - http://www.scopus.com/inward/record.url?scp=33747062417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747062417&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3083.2006.01816.x

DO - 10.1111/j.1365-3083.2006.01816.x

M3 - Article

C2 - 16918703

AN - SCOPUS:33747062417

VL - 64

SP - 336

EP - 344

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 3

ER -