Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts

R. Gáspár, A. Freek Weidema, Z. Krasznai, Peter J. Nijweide, Dirk L. Ypey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28±4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59±6 mV with a steepness factor of 0.11±0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 <15 nM) but not by verapamil (≤100μM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.

Original languageEnglish
Pages (from-to)596-598
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume430
Issue number4
DOIs
Publication statusPublished - Aug 1995

Fingerprint

Tetrodotoxin
Osteoclasts
Chickens
Depolarization
Clamping devices
Electric potential
Patch-Clamp Techniques
Verapamil
Sodium

Keywords

  • Na-current
  • osteoclast
  • Patch-clamp technique
  • Tetrodotoxin

ASJC Scopus subject areas

  • Physiology

Cite this

Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts. / Gáspár, R.; Weidema, A. Freek; Krasznai, Z.; Nijweide, Peter J.; Ypey, Dirk L.

In: Pflugers Archiv European Journal of Physiology, Vol. 430, No. 4, 08.1995, p. 596-598.

Research output: Contribution to journalArticle

Gáspár, R. ; Weidema, A. Freek ; Krasznai, Z. ; Nijweide, Peter J. ; Ypey, Dirk L. / Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts. In: Pflugers Archiv European Journal of Physiology. 1995 ; Vol. 430, No. 4. pp. 596-598.
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