Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts

Rezso Gáspár, A. Freek Weidema, Zoltán Krasznai, Peter J. Nijweide, Dirk L. Ypey

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28±4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59±6 mV with a steepness factor of 0.11±0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 < 15 nM) but not by verapamil (≤100μM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.

Original languageEnglish
Pages (from-to)596-598
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume430
Issue number4
DOIs
Publication statusPublished - Aug 1 1995

Keywords

  • Na-current
  • Patch-clamp technique
  • Tetrodotoxin
  • osteoclast

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Tetrodotoxin-sensitive fast Na<sup>+</sup> current in embryonic chicken osteoclasts'. Together they form a unique fingerprint.

  • Cite this