Tetrahydrobiopterin improves cardiac and pulmonary function after cardiopulmonary bypass

Gábor Szabó, Leila Seres, Pál Soós, Matthias Gorenflo, Béla Merkely, Ferenc Horkay, Matthias Karck, Tamás Radovits

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: Tetrahydrobiopterin (BH4) is an important cofactor of endogenous nitric oxide synthesis. In the present preclinical study, we investigated the effects of BH4 on cardiac and pulmonary function during early reperfusion in an experimental model of cardioplegic arrest and extracorporal circulation. Methods: Twelve anesthetized dogs underwent hypothermic cardiopulmonary bypass. After 60min of hypothermic cardiac arrest, reperfusion was started after application of either saline vehicle (control, n=6), or BH4 (n=6). Left-ventricular end-systolic pressure volume relationship (E es) was measured by a combined pressure-volume conductance catheter at baseline and after 60min of reperfusion. Left anterior descending (LAD) coronary (CBF) and pulmonary blood flow (PBF), endothelium-dependent vasodilatation to acetylcholine (ACh), endothelium-independent vasodilatation to sodium nitroprusside (SNP) and alveolo-arterial O 2 gradient were determined. Results: The administration of BH4 led to a significantly better recovery of E es (given as percent of baseline: 85±22 vs 46±15%, p-1, p2 gradient was significantly lower after BH4 supplementation (80±15 vs 49±14mmHg, p

Original languageEnglish
Pages (from-to)695-700
Number of pages6
JournalEuropean Journal of Cardio-thoracic Surgery
Volume40
Issue number3
DOIs
Publication statusPublished - Sep 2011

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Cardiopulmonary Bypass
Reperfusion
Vasodilation
Lung
Endothelium
Nitroprusside
Heart Arrest
Stroke Volume
Acetylcholine
Nitric Oxide
Theoretical Models
Catheters
Dogs
Blood Pressure
Pressure
sapropterin

Keywords

  • Cardiopulmonary bypass
  • Endothelial function
  • Ischemia/reperfusion injury
  • Tetrahydrobiopterin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Pulmonary and Respiratory Medicine

Cite this

Tetrahydrobiopterin improves cardiac and pulmonary function after cardiopulmonary bypass. / Szabó, Gábor; Seres, Leila; Soós, Pál; Gorenflo, Matthias; Merkely, Béla; Horkay, Ferenc; Karck, Matthias; Radovits, Tamás.

In: European Journal of Cardio-thoracic Surgery, Vol. 40, No. 3, 09.2011, p. 695-700.

Research output: Contribution to journalArticle

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