Tetra-butyl ammonium attenuates evoked release of acetylcholine from mouse hemidiaphragm preparation

H. Miyakawa, H. Iwasaka, T. Zelles, T. Noguchi

Research output: Contribution to journalArticle

1 Citation (Scopus)


Tetra-butyl ammonium is a homologous member of mono-quaternary ammonium salts, and it has been reported to have a property of nondepolarizing neuromuscular relaxant. However, no neurochemical evidences exist that tetra-butyl ammonium may interfere with quantal release of acetylcholine from motor nerve terminals. In this study, using the neurochemical method, we investigated the effect of tetra-butyl ammonium on stimulation-evoked release of acetylcholine from mouse hemidiaphragm preparation. The preparation was loaded with [3H]choline (5 μCi/ml). Low concentrations of tetra-butyl ammonium (10-5 M) had no effects. On the other hand, at concentrations of 4×10-5 and 10-4 M, this compound significantly reduced the [Ca2+]o-dependent release of acetylcholine from phrenic nerves. This finding indicates that tetra-butyl ammonium possesses a presynaptic inhibitory effect on acetylcholine release from the phrenic nerve terminal.

Original languageEnglish
Pages (from-to)517-519
Number of pages3
JournalBrain Research Bulletin
Issue number6
Publication statusPublished - Dec 1 2001


  • Acetylcholine release
  • Phrenic nerve
  • Presynaptic effect
  • Tetra-butyl ammonium

ASJC Scopus subject areas

  • Neuroscience(all)

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