Ternary complex formation between DNA-adenovirus core protein VII and TAF-Iβ/SET, an acidic molecular chaperone

Hirohito Haruki, Bela Gyurcsik, Mitsuru Okuwaki, Kyosuke Nagata

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44 Citations (Scopus)


The adenovirus (Ad) genome complexed with viral core proteins designated Ad core is the template for transcription of early genes and the first round of replication in Ad-infected cells. A cellular protein designated template-activating factor-I (TAF-I) is found to be involved in remodeling of the Ad core in vitro. Here we found that TAF-I interacts with the Ad DNA through core protein VII in infected cells in early phases of infection. In vitro binding assays using recombinant proteins showed that TAF-I forms ternary complexes with DNA-protein VII complexes.

Original languageEnglish
Pages (from-to)521-527
Number of pages7
JournalFEBS letters
Issue number3
Publication statusPublished - Dec 18 2003



  • Adenovirus
  • Chromatin immunoprecipitation
  • Histone chaperone
  • Replication
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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