Temporal alteration of spreading depression by the glycine transporter type-1 inhibitors NFPS and Org-24461 in chicken retina

Szabolcs Kertesz, Geza Szabo, Szabolcs Udvari, Gyorgy Levay, Peter Matyus, Laszlo G. Harsing

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Abstract

We used isolated chicken retina to induce spreading depression by the glutamate receptor agonist N-methyl-d-aspartate. The N-methyl-d-aspartate- induced latency time of spreading depression was extended by the glycine B binding site competitive antagonist 7-chlorokynurenic acid. Addition of the glycine transporter type-1 inhibitors NFPS and Org-24461 reversed the inhibitory effect of 7-chlorokynurenic acid on N-methyl-d- aspartate-evoked spreading depression. The glycine uptake inhibitory activity of Org-24461, NFPS, and some newly synthesized analogs of NFPS was determined in CHO cells stably expressing human glycine transporter type-1b isoform. Compounds, which failed to inhibit glycine transporter type-1, also did not have effect on retinal spreading depression. These experiments indicate that the spreading depression model in chicken retina is a useful in vitro test to determine activity of glycine transporter type-1 inhibitors. In addition, our data serve further evidence for the role of glycine transporter type-1 in retinal neurotransmission and light processing.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalBrain research
Volume1492
DOIs
Publication statusPublished - Jan 25 2013

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Keywords

  • Chicken retina
  • Glycine transporter inhibitors
  • Glycine transporter type-1
  • N-methyl-d-aspartate receptors
  • Spreading depression

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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