Temperature jump study of charge translocation during the bacteriorhodopsin photocycle

Hans Jürgen Butt, Klaus Fendler, A. Dér, Ernst Bamberg

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Temperature jump experiments were carried out on purple membranes oriented and fixed in polyacrylamide gel. With green background illumination a relaxation of the photocurrent after an infrared laser pulse could be observed. To simulate the temperature jump signals different models of the bacteriorhodopsin photocycle were tested. The parameters of these models were obtained by measuring absorbance changes and photocurrent after excitation with a 575-nm laser flash. A model with a temperature-dependent branching before the M state turned out to be satisfying. Other models, especially those with a late branching or without branching, could not reproduce the temperature jump measurements.

Original languageEnglish
Pages (from-to)851-859
Number of pages9
JournalBiophysical Journal
Volume56
Issue number5
DOIs
Publication statusPublished - Jan 1 1989

Fingerprint

Bacteriorhodopsins
Temperature
Lasers
Purple Membrane
Lighting

ASJC Scopus subject areas

  • Biophysics

Cite this

Temperature jump study of charge translocation during the bacteriorhodopsin photocycle. / Butt, Hans Jürgen; Fendler, Klaus; Dér, A.; Bamberg, Ernst.

In: Biophysical Journal, Vol. 56, No. 5, 01.01.1989, p. 851-859.

Research output: Contribution to journalArticle

Butt, Hans Jürgen ; Fendler, Klaus ; Dér, A. ; Bamberg, Ernst. / Temperature jump study of charge translocation during the bacteriorhodopsin photocycle. In: Biophysical Journal. 1989 ; Vol. 56, No. 5. pp. 851-859.
@article{bcef97d32c9947c3877a66d426d57351,
title = "Temperature jump study of charge translocation during the bacteriorhodopsin photocycle",
abstract = "Temperature jump experiments were carried out on purple membranes oriented and fixed in polyacrylamide gel. With green background illumination a relaxation of the photocurrent after an infrared laser pulse could be observed. To simulate the temperature jump signals different models of the bacteriorhodopsin photocycle were tested. The parameters of these models were obtained by measuring absorbance changes and photocurrent after excitation with a 575-nm laser flash. A model with a temperature-dependent branching before the M state turned out to be satisfying. Other models, especially those with a late branching or without branching, could not reproduce the temperature jump measurements.",
author = "Butt, {Hans J{\"u}rgen} and Klaus Fendler and A. D{\'e}r and Ernst Bamberg",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/S0006-3495(89)82731-6",
language = "English",
volume = "56",
pages = "851--859",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "5",

}

TY - JOUR

T1 - Temperature jump study of charge translocation during the bacteriorhodopsin photocycle

AU - Butt, Hans Jürgen

AU - Fendler, Klaus

AU - Dér, A.

AU - Bamberg, Ernst

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Temperature jump experiments were carried out on purple membranes oriented and fixed in polyacrylamide gel. With green background illumination a relaxation of the photocurrent after an infrared laser pulse could be observed. To simulate the temperature jump signals different models of the bacteriorhodopsin photocycle were tested. The parameters of these models were obtained by measuring absorbance changes and photocurrent after excitation with a 575-nm laser flash. A model with a temperature-dependent branching before the M state turned out to be satisfying. Other models, especially those with a late branching or without branching, could not reproduce the temperature jump measurements.

AB - Temperature jump experiments were carried out on purple membranes oriented and fixed in polyacrylamide gel. With green background illumination a relaxation of the photocurrent after an infrared laser pulse could be observed. To simulate the temperature jump signals different models of the bacteriorhodopsin photocycle were tested. The parameters of these models were obtained by measuring absorbance changes and photocurrent after excitation with a 575-nm laser flash. A model with a temperature-dependent branching before the M state turned out to be satisfying. Other models, especially those with a late branching or without branching, could not reproduce the temperature jump measurements.

UR - http://www.scopus.com/inward/record.url?scp=0000723595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000723595&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(89)82731-6

DO - 10.1016/S0006-3495(89)82731-6

M3 - Article

AN - SCOPUS:0000723595

VL - 56

SP - 851

EP - 859

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 5

ER -