Temperature dependent structural changes of intraphage T7 DNA

A. Fekete, G. Rontó, L. A. Feigin, V. V. Tikhonychev, K. Módos

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The phenomena connected with the first phase transition step of the native T7 phage at 40‡C-65‡ C have been studied using various methods. In this temperature range a) the optical melting curve shows an absorption decrease, b) the maximum of the small-angle X-ray scattering characteristic for DNA packing disappears, c) there is a drop of biological activity and d) there are changes in the structure of the difference absorption spectra of native phages versus isolated DNA. All data are interpreted assuming a structural change of the DNA due to the release of its protein coat towards the end of the first phase transition step (at 60‡-65‡ C in the case of M9 buffer). Above this temperature the intraphage DNA packing appears to be destroyed and the DNA structure seems to be similar to that in DNA solution.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBiophysics of Structure and Mechanism
Volume9
Issue number1
DOIs
Publication statusPublished - Mar 1982

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Temperature
DNA
Phase Transition
Bacteriophage T7
Capsid Proteins
Bacteriophages
Freezing
Buffers
X-Rays

Keywords

  • Bacteriophages
  • Optical melting curve
  • Phase transition
  • X-ray scattering

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Medicine(all)

Cite this

Temperature dependent structural changes of intraphage T7 DNA. / Fekete, A.; Rontó, G.; Feigin, L. A.; Tikhonychev, V. V.; Módos, K.

In: Biophysics of Structure and Mechanism, Vol. 9, No. 1, 03.1982, p. 1-9.

Research output: Contribution to journalArticle

Fekete, A. ; Rontó, G. ; Feigin, L. A. ; Tikhonychev, V. V. ; Módos, K. / Temperature dependent structural changes of intraphage T7 DNA. In: Biophysics of Structure and Mechanism. 1982 ; Vol. 9, No. 1. pp. 1-9.
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