Temperature dependence of bovine serum albumin adsorption onto a poly(ethylene oxide)-grafted surface

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Abstract

The adsorption of bovine serum albumin (BSA) onto bare polyethylene (PE) and poly(ethylene oxide)-grafted PE particles was studied by the solution depletion method. Adsorption measurements were performed at different temperatures in the range of 25-65°C. The PE surface adsorbs high amounts of protein corresponding to a complete side-on monolayer in the temperature range 25-45°C. The considerable increase in the adsorbed amount and the shape of the isotherms at temperatures above 55°C indicate surface aggregation approaching the denaturation temperature of BSA. A lower degree of protein adsorption was obtained for PEO-grafted surfaces, and the amount of BSA adsorbed increases continuously with the temperature over the whole range studied. This is in accordance with the changing wettability of the PEO-grafted surface with temperature. The different temperature dependences of the adsorption behaviour found for PE and PEO-grafted surfaces show the joint influence of the protein properties in solution and the surface interactions on the adsorption.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume76
Issue numberC
DOIs
Publication statusPublished - Sep 8 1993

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Bovine Serum Albumin
ethylene oxide
Polyethylene oxides
albumins
serums
Adsorption
temperature dependence
Polyethylene
adsorption
polyethylenes
Polyethylenes
proteins
Temperature
temperature
Proteins
biopolymer denaturation
Denaturation
wettability
surface reactions
isotherms

Keywords

  • Adsorption
  • bovine serum albumin
  • poly(ethylene oxide)
  • temperature effect.

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

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title = "Temperature dependence of bovine serum albumin adsorption onto a poly(ethylene oxide)-grafted surface",
abstract = "The adsorption of bovine serum albumin (BSA) onto bare polyethylene (PE) and poly(ethylene oxide)-grafted PE particles was studied by the solution depletion method. Adsorption measurements were performed at different temperatures in the range of 25-65°C. The PE surface adsorbs high amounts of protein corresponding to a complete side-on monolayer in the temperature range 25-45°C. The considerable increase in the adsorbed amount and the shape of the isotherms at temperatures above 55°C indicate surface aggregation approaching the denaturation temperature of BSA. A lower degree of protein adsorption was obtained for PEO-grafted surfaces, and the amount of BSA adsorbed increases continuously with the temperature over the whole range studied. This is in accordance with the changing wettability of the PEO-grafted surface with temperature. The different temperature dependences of the adsorption behaviour found for PE and PEO-grafted surfaces show the joint influence of the protein properties in solution and the surface interactions on the adsorption.",
keywords = "Adsorption, bovine serum albumin, poly(ethylene oxide), temperature effect.",
author = "E. Kiss",
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T1 - Temperature dependence of bovine serum albumin adsorption onto a poly(ethylene oxide)-grafted surface

AU - Kiss, E.

PY - 1993/9/8

Y1 - 1993/9/8

N2 - The adsorption of bovine serum albumin (BSA) onto bare polyethylene (PE) and poly(ethylene oxide)-grafted PE particles was studied by the solution depletion method. Adsorption measurements were performed at different temperatures in the range of 25-65°C. The PE surface adsorbs high amounts of protein corresponding to a complete side-on monolayer in the temperature range 25-45°C. The considerable increase in the adsorbed amount and the shape of the isotherms at temperatures above 55°C indicate surface aggregation approaching the denaturation temperature of BSA. A lower degree of protein adsorption was obtained for PEO-grafted surfaces, and the amount of BSA adsorbed increases continuously with the temperature over the whole range studied. This is in accordance with the changing wettability of the PEO-grafted surface with temperature. The different temperature dependences of the adsorption behaviour found for PE and PEO-grafted surfaces show the joint influence of the protein properties in solution and the surface interactions on the adsorption.

AB - The adsorption of bovine serum albumin (BSA) onto bare polyethylene (PE) and poly(ethylene oxide)-grafted PE particles was studied by the solution depletion method. Adsorption measurements were performed at different temperatures in the range of 25-65°C. The PE surface adsorbs high amounts of protein corresponding to a complete side-on monolayer in the temperature range 25-45°C. The considerable increase in the adsorbed amount and the shape of the isotherms at temperatures above 55°C indicate surface aggregation approaching the denaturation temperature of BSA. A lower degree of protein adsorption was obtained for PEO-grafted surfaces, and the amount of BSA adsorbed increases continuously with the temperature over the whole range studied. This is in accordance with the changing wettability of the PEO-grafted surface with temperature. The different temperature dependences of the adsorption behaviour found for PE and PEO-grafted surfaces show the joint influence of the protein properties in solution and the surface interactions on the adsorption.

KW - Adsorption

KW - bovine serum albumin

KW - poly(ethylene oxide)

KW - temperature effect.

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