Technique for study of hypothalamus - pituitary - testis function in vitro

I. Vermes, E. K. Toth, G. Telegdy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

An in vitro system consisting of the rat hypothalamic tissue, the collagenase-digested isolated anterior pituitary cells and the Leydig cells, has been employed, where the testosterone production of the incubated Leydig cells was measured as the end point. The method appeared to be highly sensitive and specific in studying the functional interrelations of hypothalamus-pituitary-testis system. The sensitivity of the Leydig cells to hCG was 0.1-1.0 mU per 106 cells, and that of the anterior pituitary cells to LHRH responding with LH release was in the range of 0.1-1.0 ng per 106 cells. Two hypothalamic blocks can release a sufficient amount of LHRH to produce LH secretion in the pituitary cell preparation. The described technique seems to be a suitable in vitro approach for measuring the functional activity of the system studied and for evaluation of the sites of response to various external agents.

Original languageEnglish
Title of host publicationEndocrinologia Experimentalis
Pages97-103
Number of pages7
Volume13
Edition2
Publication statusPublished - 1979

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Hypothalamus
Testis
Leydig Cells
Gonadotropin-Releasing Hormone
Collagenases
Testosterone
In Vitro Techniques

ASJC Scopus subject areas

  • Endocrinology

Cite this

Vermes, I., Toth, E. K., & Telegdy, G. (1979). Technique for study of hypothalamus - pituitary - testis function in vitro. In Endocrinologia Experimentalis (2 ed., Vol. 13, pp. 97-103)

Technique for study of hypothalamus - pituitary - testis function in vitro. / Vermes, I.; Toth, E. K.; Telegdy, G.

Endocrinologia Experimentalis. Vol. 13 2. ed. 1979. p. 97-103.

Research output: Chapter in Book/Report/Conference proceedingChapter

Vermes, I, Toth, EK & Telegdy, G 1979, Technique for study of hypothalamus - pituitary - testis function in vitro. in Endocrinologia Experimentalis. 2 edn, vol. 13, pp. 97-103.
Vermes I, Toth EK, Telegdy G. Technique for study of hypothalamus - pituitary - testis function in vitro. In Endocrinologia Experimentalis. 2 ed. Vol. 13. 1979. p. 97-103
Vermes, I. ; Toth, E. K. ; Telegdy, G. / Technique for study of hypothalamus - pituitary - testis function in vitro. Endocrinologia Experimentalis. Vol. 13 2. ed. 1979. pp. 97-103
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