Technique-dependent variations in cerebral microvessel blood volumes and hematocrits in the rat

D. Bereczki, L. Wei, V. Acuff, K. Gruber, A. Tajima, C. Patlak, J. Fenstermacher

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To quantitate small parenchymal microvessel blood volumes in the brain, the distribution spaces of radiolabeled red blood cells (RBC) and serum albumin (RISA) were assessed in rats by different methods of tissue sampling and radioassay. Three minutes after intravenous administration of 55Fe-RBCs and/or 125I-RISA, the rats were decapitated. The brain was either immediately frozen within the skull and later removed (head-frozen group) or rapidly removed from the skull and then frozen (brain-frozen group). Radioactivity was measured either by liquid scintillation counting of tissue pieces, which contained pial plus large and small parenchymal microvessels, or by quantitative autoradiography (QAR) of tissue sections, which indicated small parenchymal microvessel blood only. In 12 of 15 areas, the RISA, RBC, and blood volumes determined by liquid scintillation counting of head-frozen tissue pieces were equal to or greater than those of brain-frozen tissue; this indicated ≤25% greater blood retention in pial and parenchymal microvessels with head freezing. At the parenchymal microvessel level (QAR assay), the distribution volumes of RBCs, RISA, and blood were similar with the two freezing techniques; hence with QAR either freezing procedure can be used to assess small parenchymal microvessel blood volumes.

Original languageEnglish
Pages (from-to)918-924
Number of pages7
JournalJournal of Applied Physiology
Volume73
Issue number3
Publication statusPublished - 1992

Fingerprint

Microvessels
Hematocrit
Blood Volume
Autoradiography
Freezing
Scintillation Counting
Head
Brain
Skull
Erythrocytes
Cell Size
Serum Albumin
Intravenous Administration
Radioactivity
Cerebral Blood Volume
Serum

Keywords

  • blood retention
  • brain freezing
  • iodine-125-radiolabeled serum albumin
  • iron-55-labeled red blood cells
  • quantitative autoradiography
  • tissue sampling

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Bereczki, D., Wei, L., Acuff, V., Gruber, K., Tajima, A., Patlak, C., & Fenstermacher, J. (1992). Technique-dependent variations in cerebral microvessel blood volumes and hematocrits in the rat. Journal of Applied Physiology, 73(3), 918-924.

Technique-dependent variations in cerebral microvessel blood volumes and hematocrits in the rat. / Bereczki, D.; Wei, L.; Acuff, V.; Gruber, K.; Tajima, A.; Patlak, C.; Fenstermacher, J.

In: Journal of Applied Physiology, Vol. 73, No. 3, 1992, p. 918-924.

Research output: Contribution to journalArticle

Bereczki, D, Wei, L, Acuff, V, Gruber, K, Tajima, A, Patlak, C & Fenstermacher, J 1992, 'Technique-dependent variations in cerebral microvessel blood volumes and hematocrits in the rat', Journal of Applied Physiology, vol. 73, no. 3, pp. 918-924.
Bereczki, D. ; Wei, L. ; Acuff, V. ; Gruber, K. ; Tajima, A. ; Patlak, C. ; Fenstermacher, J. / Technique-dependent variations in cerebral microvessel blood volumes and hematocrits in the rat. In: Journal of Applied Physiology. 1992 ; Vol. 73, No. 3. pp. 918-924.
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