TBP is not universally required for zygotic RNA polymerase II transcription in zebrafish

Ferenc Müller, L. Lakatos, Jean Christophe Dantonel, Uwe Strähle, Làszlò Tora

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

General transcription factors TFIIA, B, D, E, F, H, and RNA polymerase II (Pol II) are required for accurate initiation of Pol II transcription. The TATA binding protein (TBP), a subunit of TFIID, is responsible for recognition of the TATA box, a core element shared by a category of class II promoters [1]. Recently, novel TBP-like factors (TLFs) have been described in metazoan organisms [2]. In spite of the numerous in vitro studies describing the general role of TBP in RNA polymerase II (Pol II) transcription initiation, the precise function of TBP and the newly described TLF is poorly understood in vivo. We inhibited TBP and TLF function in zebrafish embryos to study the role of these factors during zygotic transcription. A dominant-negative variant of TLF mRNA and a TBP morpholino antisense oligo was used to block either TLF or TBP function. Both TBP- or TLF-blocked embryos developed normally until the midblastula stage; however, they then failed to gastrulate. Several zygotic regulatory genes were downregulated by a block in either TBP or TLF function, while others were differentially affected. These results suggest that TBP is not universally required for Pol II transcription in vertebrates and that there is a differential requirement for TBP and TLF during early embryogenesis.

Original languageEnglish
Pages (from-to)282-287
Number of pages6
JournalCurrent Biology
Volume11
Issue number4
DOIs
Publication statusPublished - Feb 20 2001

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TATA-Box Binding Protein
RNA Polymerase II
Zebrafish
Transcription
DNA-directed RNA polymerase
Danio rerio
binding proteins
transcription (genetics)
Transcription Factor TFIIA
embryo (animal)
Embryonic Structures
Transcription Factor TFIID
General Transcription Factors
TATA box
Morpholinos
TATA Box
Protein Subunits
protein subunits
Regulator Genes
regulator genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

TBP is not universally required for zygotic RNA polymerase II transcription in zebrafish. / Müller, Ferenc; Lakatos, L.; Dantonel, Jean Christophe; Strähle, Uwe; Tora, Làszlò.

In: Current Biology, Vol. 11, No. 4, 20.02.2001, p. 282-287.

Research output: Contribution to journalArticle

Müller, Ferenc ; Lakatos, L. ; Dantonel, Jean Christophe ; Strähle, Uwe ; Tora, Làszlò. / TBP is not universally required for zygotic RNA polymerase II transcription in zebrafish. In: Current Biology. 2001 ; Vol. 11, No. 4. pp. 282-287.
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