Taurine and β-alanine act on both GABA and glycine receptors in Xenopus oocyte injected with mouse brain messenger RNA

Tetsuro Horikoshi, Atsushi Asanuma, Keiji Yanagisawa, Kaijiro Anzai, S. Goto

Research output: Contribution to journalArticle

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Abstract

The responding pathway (process from agonist binding to channel opening) of taurine and β-alanine was investigated in Xenopus oocytes injected with mouse brain poly(A)+ RNA. Responses to γ-aminobutyric acid (GABA), glycine, taurine and β-alanine were induced in oocytes injected with poly(A)+ RNA extracted from 3 regions, cerebrum, cerebellum and brainstem of the mouse brain. From comparison, responses to these 4 inhibitory amino acids in each regional poly(A)+ RNA-injected oocytes were categorized into at least 3 groups: (1) GABA, (2) glycine, and (3) taurine and β-alanine. No cross-desensitization was observed between GABA response and glycine response, but taurine and β-alanine responses cross-desensitized both the GABA and glycine responses. Taurine and β-alanine responses were partially inhibited by the GABA antagonist, bicuculline, and also by the glycine antagonist, strychnine. The results suggest that the taurine or the β-alanine response in the brain is caused through both the GABA receptor and the glycine receptor.

Original languageEnglish
Pages (from-to)97-105
Number of pages9
JournalMolecular Brain Research
Volume4
Issue number2
DOIs
Publication statusPublished - 1988

Fingerprint

Glycine Receptors
GABA Receptors
Taurine
Xenopus
Alanine
Oocytes
Glycine
gamma-Aminobutyric Acid
Messenger RNA
Brain
GABA Antagonists
Aminobutyrates
Strychnine
Bicuculline
Cerebrum
Cerebellum
Brain Stem
Amino Acids

Keywords

  • Glycine
  • Messenger ribonucleic acid
  • Mouse brain
  • Neurotransmitter receptor
  • Taurine
  • Xenopus oocyte
  • β-Alanine
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Taurine and β-alanine act on both GABA and glycine receptors in Xenopus oocyte injected with mouse brain messenger RNA. / Horikoshi, Tetsuro; Asanuma, Atsushi; Yanagisawa, Keiji; Anzai, Kaijiro; Goto, S.

In: Molecular Brain Research, Vol. 4, No. 2, 1988, p. 97-105.

Research output: Contribution to journalArticle

Horikoshi, Tetsuro ; Asanuma, Atsushi ; Yanagisawa, Keiji ; Anzai, Kaijiro ; Goto, S. / Taurine and β-alanine act on both GABA and glycine receptors in Xenopus oocyte injected with mouse brain messenger RNA. In: Molecular Brain Research. 1988 ; Vol. 4, No. 2. pp. 97-105.
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