Targets, receptors and effects of muscarinic neuromodulation on giant neurones of the rat dorsal cochlear nucleus

B. Pál, Á Koszeghy, P. Pap, G. Bakondi, K. Pocsai, G. Szücs, Z. Rusznák

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although cholinergic modulation of the cochlear nucleus (CN) is functionally important, neither its cellular consequences nor the types of receptors conveying it are precisely known. The aim of this work was to characterise the cholinergic effects on giant cells of the CN, using electrophysiology and quantitative polymerase chain reaction. Application of the cholinergic agonist carbachol increased the spontaneous activity of the giant cells; which was partly the consequence of the reduction in a K+ conductance. This effect was mediated via M4 and M3 receptors. Cholinergic modulation also affected the synaptic transmission targeting the giant cells. Excitatory synaptic currents evoked by the stimulation of the superficial and deep regions of the CN were sensitive to cholinergic modulation: the amplitude of the first postsynaptic current was reduced, and the short-term depression was also altered. These changes were mediated via M3 receptors alone and via the combination of M4, M2 and M3 receptors, when the superficial and deep layers, respectively, were activated. Inhibitory synaptic currents evoked from the superficial layer showed short-term depression, but they were unaffected by carbachol. In contrast, inhibitory currents triggered by the activation of the deep parts exhibited no significant short-term depression, but they were highly sensitive to cholinergic activation, which was mediated via M3 receptors. Our results indicate that pre- and postsynaptic muscarinic receptors mediate cholinergic modulation on giant cells. The present findings shed light on the cellular mechanisms of a tonic cholinergic modulation in the CN, which may become particularly important in evoking contralateral excitatory responses under certain pathological conditions.

Original languageEnglish
Pages (from-to)769-782
Number of pages14
JournalEuropean Journal of Neuroscience
Volume30
Issue number5
DOIs
Publication statusPublished - Sep 2009

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Cochlear Nucleus
Muscarinic Receptors
Cholinergic Agents
Neurons
Giant Cells
Carbachol
Cholinergic Agonists
Synaptic Potentials
Electrophysiology
Cell Nucleus
Synaptic Transmission
Polymerase Chain Reaction

Keywords

  • Dorsal cochlear nucleus
  • EPSCs
  • IPSCs
  • M-current
  • Muscarinic receptors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Targets, receptors and effects of muscarinic neuromodulation on giant neurones of the rat dorsal cochlear nucleus. / Pál, B.; Koszeghy, Á; Pap, P.; Bakondi, G.; Pocsai, K.; Szücs, G.; Rusznák, Z.

In: European Journal of Neuroscience, Vol. 30, No. 5, 09.2009, p. 769-782.

Research output: Contribution to journalArticle

Pál, B. ; Koszeghy, Á ; Pap, P. ; Bakondi, G. ; Pocsai, K. ; Szücs, G. ; Rusznák, Z. / Targets, receptors and effects of muscarinic neuromodulation on giant neurones of the rat dorsal cochlear nucleus. In: European Journal of Neuroscience. 2009 ; Vol. 30, No. 5. pp. 769-782.
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