Targeting mitochondrial dysfunction with L-alpha glycerylphosphorylcholine

Gerda Strifler, Eszter Tuboly, Anikó Görbe, M. Borós, Daniella Pécz, Petra Hartmann

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: We hypothesized that L-alpha-glycerylphosphorylcholine (GPC), a deacylatedphosphatidylcholine derivative, can influence the mitochondrial respiratory activity and in this way, may exert tissue protective effects. Methods: Rat liver mitochondria were examined with high-resolution respirometry to analyze the effects of GPC on the electron transport chain in normoxic and anoxic conditions. Besides, Sprague-Dawley rats were subjected to sham operation or standardized liver ischemiareperfusion (IR), with or without GPC administration. The reduced glutathione (GSH) and oxidized glutathione disulfide (GSSG), the tissue myeloperoxidase, xanthine oxidoreductase and NADPH oxidases activities were measured. Tissue malondialdehyde and nitrite/nitrate formation, together with blood superoxide and hydrogen-peroxide production were assessed. Results: GPC increased the efficacy of complex I-linked mitochondrial oxygen consumption, with significantly lower in vitro leak respiration. Mechanistically, liver IR injury was accompanied by deteriorated mitochondrial respiration and enhanced ROS production and, as a consequence, by significantly increased inflammatory enzyme activities. GPC administration decreased the inflammatory activation in line with the reduced oxidative and nitrosative stress markers. Conclusion: GPC, by preserving the mitochondrial complex I function respiration, reduced the biochemical signs of oxidative stress after an IR episode. This suggests that GPC is a mitochondriatargeted compound that indirectly suppresses the activity of major intracellular superoxidegenerating enzymes.

Original languageEnglish
Article numbere0166682
JournalPLoS One
Volume11
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

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Glycerylphosphorylcholine
NADH dehydrogenase (ubiquinone)
breathing
liver
glutathione
Glutathione Disulfide
xanthine
myeloperoxidase
Liver
normoxia
rats
oxidoreductases
electron transport chain
Respiration
sulfides
anaerobic conditions
superoxide anion
nitrites
malondialdehyde
oxygen consumption

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Targeting mitochondrial dysfunction with L-alpha glycerylphosphorylcholine. / Strifler, Gerda; Tuboly, Eszter; Görbe, Anikó; Borós, M.; Pécz, Daniella; Hartmann, Petra.

In: PLoS One, Vol. 11, No. 11, e0166682, 01.11.2016.

Research output: Contribution to journalArticle

Strifler, Gerda ; Tuboly, Eszter ; Görbe, Anikó ; Borós, M. ; Pécz, Daniella ; Hartmann, Petra. / Targeting mitochondrial dysfunction with L-alpha glycerylphosphorylcholine. In: PLoS One. 2016 ; Vol. 11, No. 11.
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