Targeting and translocation of the phosphate carrier/p32 to the inner membrane of yeast mitochondria

Klaus Dietmeier, Vincenzo Zara, Annamaria Palmisano, Ferdinando Palmier, Wolfgang Voos, Jens Schlossmann, Martin Moczko, G. Kispál, Nikolaus Pfanner

Research output: Contribution to journalArticle

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Abstract

We analyzed the submitochondrial location and biogenesis pathway of the phosphate carrier (PiC), also termed p32, of Saccharomyces cerevisiae mitochondria. PiC/p32 was found to behave as an integral membrane protein that cofractionated with the ADP/ATP carrier of the inner membrane. Import of the precursor of PiC/p32 required a membrane potential across the inner membrane, supporting its localization to the inner membrane. This makes it unlikely that the major function of PiC/p32 is that of an import receptor on the surface of the mitochondrial outer membrane. Furthermore, we found that both receptors MOM72 and MOM19 were involved in the import pathway of the precursor of PiC/p32 with MOM72 being responsible for the bulk of import. Yeast PiC/p32 is thus not only structurally homologous to the ADP/ATP carrier, but has a similar targeting mechanism and submitochondrial location, supporting its classification as a member of the inner membrane carrier family.

Original languageEnglish
Pages (from-to)25958-25964
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number34
Publication statusPublished - Dec 5 1993

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Phosphate Transport Proteins
Mitochondria
Yeast
Yeasts
ATP Translocases Mitochondrial ADP
Membranes
Mitochondrial Membranes
Membrane Potentials
Saccharomyces cerevisiae
Membrane Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dietmeier, K., Zara, V., Palmisano, A., Palmier, F., Voos, W., Schlossmann, J., ... Pfanner, N. (1993). Targeting and translocation of the phosphate carrier/p32 to the inner membrane of yeast mitochondria. Journal of Biological Chemistry, 268(34), 25958-25964.

Targeting and translocation of the phosphate carrier/p32 to the inner membrane of yeast mitochondria. / Dietmeier, Klaus; Zara, Vincenzo; Palmisano, Annamaria; Palmier, Ferdinando; Voos, Wolfgang; Schlossmann, Jens; Moczko, Martin; Kispál, G.; Pfanner, Nikolaus.

In: Journal of Biological Chemistry, Vol. 268, No. 34, 05.12.1993, p. 25958-25964.

Research output: Contribution to journalArticle

Dietmeier, K, Zara, V, Palmisano, A, Palmier, F, Voos, W, Schlossmann, J, Moczko, M, Kispál, G & Pfanner, N 1993, 'Targeting and translocation of the phosphate carrier/p32 to the inner membrane of yeast mitochondria', Journal of Biological Chemistry, vol. 268, no. 34, pp. 25958-25964.
Dietmeier K, Zara V, Palmisano A, Palmier F, Voos W, Schlossmann J et al. Targeting and translocation of the phosphate carrier/p32 to the inner membrane of yeast mitochondria. Journal of Biological Chemistry. 1993 Dec 5;268(34):25958-25964.
Dietmeier, Klaus ; Zara, Vincenzo ; Palmisano, Annamaria ; Palmier, Ferdinando ; Voos, Wolfgang ; Schlossmann, Jens ; Moczko, Martin ; Kispál, G. ; Pfanner, Nikolaus. / Targeting and translocation of the phosphate carrier/p32 to the inner membrane of yeast mitochondria. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 34. pp. 25958-25964.
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