Targeted isolation of nonTIR-NBS-LRR resistance gene analogs in apricot

A. Gutermuth, A. Pedryc

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two distinct families of Coiled-Coil Nucleotide Binding Site Leucin Rich Repeat (CC-NBS-LRR or nonTIR-NBS-LRR) resistance gene analogs were targeted identified from apricot (Prunus armeniaca L.) cultivar 'Goldrich'. We developed a PCR approach that can successfully use nonTIR-NBS-LRR RGA amplification. In case of the apricot the previously published degenerate primers designed to the conservative regions of the NBS domain amplified only RGA sequences, which belong to the TIR-NBS-LRR group. Based on the alignment the NBS regions of 28 resistance genes from the two divided groups two selective CC-NBS-LRR primers were designed to the kinase 2 and the hydrophobic motifs. We amplified the region between the P-loop and the hydrophobic motifs, and PCR products were cloned. The clones were screened for nonTIR-NBS-LRRs with semi-nested PCR using the degenerate primer designed for the kinase-2 domain. This motif contains a unique trypthophan on the C-terminus highly specific to nonTIR-NBS-LRRs. From the putative NBS-LRR PCR products tested so far, 20% were positive for the nonTIR-specific motif. After sequencing and translating of the positives clones it led us to the identification of seven unique amino acid sequences into two distinct families of resistance gene analogs. Based on these preliminary results, this approach seems to be feasible to isolate nonTIR NBS-LRR RGAs from apricot.

Original languageEnglish
Title of host publicationActa Horticulturae
PublisherInternational Society for Horticultural Science
Pages531-535
Number of pages5
ISBN (Print)9789066056121
DOIs
Publication statusPublished - 2009

Publication series

NameActa Horticulturae
Volume839
ISSN (Print)0567-7572

Keywords

  • Apricot (Prunus armeniaca L.)
  • NonTIR-NBS-LRR
  • Resistance gene analog (RGA)

ASJC Scopus subject areas

  • Horticulture

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  • Cite this

    Gutermuth, A., & Pedryc, A. (2009). Targeted isolation of nonTIR-NBS-LRR resistance gene analogs in apricot. In Acta Horticulturae (pp. 531-535). (Acta Horticulturae; Vol. 839). International Society for Horticultural Science. https://doi.org/10.17660/actahortic.2009.839.72