Szteroidok tomegspektrometrias szerkezetvizsgalata kulonbozo ionizacios modszerek es MS/MS technikak kombinalt alkalmazasaval

Translated title of the contribution: Tandem MS combined with different ionisation techniques in the structural study of steroids

Mák Marianna, Francsicsné Czinege Erzsébet, Mahó Sándor, Z. Tuba

Research output: Contribution to journalArticle

Abstract

The various mass spectrometric ionisation techniques involve different ionisation energy. Their parallel application may provide complementary information for the structure. By the use of soft ionisation methods, especially when combined with tandem mass spectrometric techniques, stereospecific dissociation can also be detected, as the fragmentation processes of low energy differences result in significant abundance differences. The EI spectra of the stereoisomer compounds 1 and 2 are compared to their FAB-daughter ion spectra. This latter method leads to higher differences in the water elimination, indicating that the β-hydroxi- isomer is more stable. ESI studies, using the 'in-source CID' technique and also combined with MS/MS investigation have provided direct information about the position of substituents for the bis-quaternary salts of amino androstane derivatives, pipecuromium bromide [ARDUAN (3)] and its related compound 4. Due to the complementary information of the different ionisation methods used in mass spectrometry, the exact and detailed structural study requires the combined application of the various ionisation techniques together with MS/MS methods.

Original languageHungarian
Pages (from-to)181-187
Number of pages7
JournalActa Pharmaceutica Hungarica
Volume69
Issue number4
Publication statusPublished - Sep 1999

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Steroids
Stereoisomerism
Bromides
Mass Spectrometry
Salts
Ions
Water

ASJC Scopus subject areas

  • Pharmaceutical Science

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Szteroidok tomegspektrometrias szerkezetvizsgalata kulonbozo ionizacios modszerek es MS/MS technikak kombinalt alkalmazasaval. / Marianna, Mák; Erzsébet, Francsicsné Czinege; Sándor, Mahó; Tuba, Z.

In: Acta Pharmaceutica Hungarica, Vol. 69, No. 4, 09.1999, p. 181-187.

Research output: Contribution to journalArticle

Marianna, Mák ; Erzsébet, Francsicsné Czinege ; Sándor, Mahó ; Tuba, Z. / Szteroidok tomegspektrometrias szerkezetvizsgalata kulonbozo ionizacios modszerek es MS/MS technikak kombinalt alkalmazasaval. In: Acta Pharmaceutica Hungarica. 1999 ; Vol. 69, No. 4. pp. 181-187.
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