Tandem mass spectrometric study of ciprofloxacin-poly(ethylene glycol) conjugate in the presence of alkali metal ions

S. Kéki, Lajos Nagy, Ákos Kuki, Gábor Pintér, P. Herczegh, M. Zsuga

Research output: Contribution to journalArticle

Abstract

The fragmentation and fragmentation behaviors of singly, doubly, and triply charged adducts of ciprofloxacin-poly(ethylene glycol) conjugate (P_Cf) with alkali metal ions, including Li+, Na+ and K+ ions, generated by electrospray (ESI) were studied as a function of collision energy. The product ion spectra of adducts with charge states +1, +2, and +3 are dominated by product ions arising from the loss-neutral moiety (ciprofloxacin) and CO2, and ions formed by dissociation of the precursor ion ([P_Cf + xM]x+) into product ions [P + (x - 1)M](x-1)+ and [Cf + M]+ (where P_Cf, P and Cf represent the ciprofloxacin-poly(ethylene glycol) conjugate, the poly(ethylene glycol) backbone without the endgroups, and the ciprofloxacin moiety, respectively; M is the alkali metal ion and x is the charge). It was found that the metal ions do not significantly alter the fragmentation pattern of ciprofloxacin-poly(ethylene glycol) conjugate. It is also interesting that the run and the shape of the survival yield curves for the singly and doubly charged adduct ions are independent of the cation. However, in the case of triply charged adducts, survival yield curves follow each other in the order K+, Na+, and Li+. Based on the experimental results, a fragmentation mechanism for the singly and multiply charged adducts of P_Cf with alkali metal ions is given. In addition, a tentative description of the signal intensity variations of the product ions with the lab frame collision energy is also reported.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalInternational Journal of Mass Spectrometry
Volume275
Issue number1-3
DOIs
Publication statusPublished - Aug 1 2008

Fingerprint

Alkali Metals
Alkali metals
Ciprofloxacin
alkali metals
Polyethylene glycols
Metal ions
glycols
metal ions
ethylene
Ions
adducts
fragmentation
ions
products
collisions
curves
Byproducts
Cations
Positive ions
dissociation

Keywords

  • Cation attachment
  • Ciprofloxacin-poly(ethylene glycol) conjugate
  • Energy-variable collision-induced dissociation
  • Fragmentation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy

Cite this

Tandem mass spectrometric study of ciprofloxacin-poly(ethylene glycol) conjugate in the presence of alkali metal ions. / Kéki, S.; Nagy, Lajos; Kuki, Ákos; Pintér, Gábor; Herczegh, P.; Zsuga, M.

In: International Journal of Mass Spectrometry, Vol. 275, No. 1-3, 01.08.2008, p. 104-109.

Research output: Contribution to journalArticle

@article{a68bfd52c8844b83a29f4b2192b72cd0,
title = "Tandem mass spectrometric study of ciprofloxacin-poly(ethylene glycol) conjugate in the presence of alkali metal ions",
abstract = "The fragmentation and fragmentation behaviors of singly, doubly, and triply charged adducts of ciprofloxacin-poly(ethylene glycol) conjugate (P_Cf) with alkali metal ions, including Li+, Na+ and K+ ions, generated by electrospray (ESI) were studied as a function of collision energy. The product ion spectra of adducts with charge states +1, +2, and +3 are dominated by product ions arising from the loss-neutral moiety (ciprofloxacin) and CO2, and ions formed by dissociation of the precursor ion ([P_Cf + xM]x+) into product ions [P + (x - 1)M](x-1)+ and [Cf + M]+ (where P_Cf, P and Cf represent the ciprofloxacin-poly(ethylene glycol) conjugate, the poly(ethylene glycol) backbone without the endgroups, and the ciprofloxacin moiety, respectively; M is the alkali metal ion and x is the charge). It was found that the metal ions do not significantly alter the fragmentation pattern of ciprofloxacin-poly(ethylene glycol) conjugate. It is also interesting that the run and the shape of the survival yield curves for the singly and doubly charged adduct ions are independent of the cation. However, in the case of triply charged adducts, survival yield curves follow each other in the order K+, Na+, and Li+. Based on the experimental results, a fragmentation mechanism for the singly and multiply charged adducts of P_Cf with alkali metal ions is given. In addition, a tentative description of the signal intensity variations of the product ions with the lab frame collision energy is also reported.",
keywords = "Cation attachment, Ciprofloxacin-poly(ethylene glycol) conjugate, Energy-variable collision-induced dissociation, Fragmentation",
author = "S. K{\'e}ki and Lajos Nagy and {\'A}kos Kuki and G{\'a}bor Pint{\'e}r and P. Herczegh and M. Zsuga",
year = "2008",
month = "8",
day = "1",
doi = "10.1016/j.ijms.2008.05.032",
language = "English",
volume = "275",
pages = "104--109",
journal = "International Journal of Mass Spectrometry",
issn = "1387-3806",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Tandem mass spectrometric study of ciprofloxacin-poly(ethylene glycol) conjugate in the presence of alkali metal ions

AU - Kéki, S.

AU - Nagy, Lajos

AU - Kuki, Ákos

AU - Pintér, Gábor

AU - Herczegh, P.

AU - Zsuga, M.

PY - 2008/8/1

Y1 - 2008/8/1

N2 - The fragmentation and fragmentation behaviors of singly, doubly, and triply charged adducts of ciprofloxacin-poly(ethylene glycol) conjugate (P_Cf) with alkali metal ions, including Li+, Na+ and K+ ions, generated by electrospray (ESI) were studied as a function of collision energy. The product ion spectra of adducts with charge states +1, +2, and +3 are dominated by product ions arising from the loss-neutral moiety (ciprofloxacin) and CO2, and ions formed by dissociation of the precursor ion ([P_Cf + xM]x+) into product ions [P + (x - 1)M](x-1)+ and [Cf + M]+ (where P_Cf, P and Cf represent the ciprofloxacin-poly(ethylene glycol) conjugate, the poly(ethylene glycol) backbone without the endgroups, and the ciprofloxacin moiety, respectively; M is the alkali metal ion and x is the charge). It was found that the metal ions do not significantly alter the fragmentation pattern of ciprofloxacin-poly(ethylene glycol) conjugate. It is also interesting that the run and the shape of the survival yield curves for the singly and doubly charged adduct ions are independent of the cation. However, in the case of triply charged adducts, survival yield curves follow each other in the order K+, Na+, and Li+. Based on the experimental results, a fragmentation mechanism for the singly and multiply charged adducts of P_Cf with alkali metal ions is given. In addition, a tentative description of the signal intensity variations of the product ions with the lab frame collision energy is also reported.

AB - The fragmentation and fragmentation behaviors of singly, doubly, and triply charged adducts of ciprofloxacin-poly(ethylene glycol) conjugate (P_Cf) with alkali metal ions, including Li+, Na+ and K+ ions, generated by electrospray (ESI) were studied as a function of collision energy. The product ion spectra of adducts with charge states +1, +2, and +3 are dominated by product ions arising from the loss-neutral moiety (ciprofloxacin) and CO2, and ions formed by dissociation of the precursor ion ([P_Cf + xM]x+) into product ions [P + (x - 1)M](x-1)+ and [Cf + M]+ (where P_Cf, P and Cf represent the ciprofloxacin-poly(ethylene glycol) conjugate, the poly(ethylene glycol) backbone without the endgroups, and the ciprofloxacin moiety, respectively; M is the alkali metal ion and x is the charge). It was found that the metal ions do not significantly alter the fragmentation pattern of ciprofloxacin-poly(ethylene glycol) conjugate. It is also interesting that the run and the shape of the survival yield curves for the singly and doubly charged adduct ions are independent of the cation. However, in the case of triply charged adducts, survival yield curves follow each other in the order K+, Na+, and Li+. Based on the experimental results, a fragmentation mechanism for the singly and multiply charged adducts of P_Cf with alkali metal ions is given. In addition, a tentative description of the signal intensity variations of the product ions with the lab frame collision energy is also reported.

KW - Cation attachment

KW - Ciprofloxacin-poly(ethylene glycol) conjugate

KW - Energy-variable collision-induced dissociation

KW - Fragmentation

UR - http://www.scopus.com/inward/record.url?scp=47549084041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47549084041&partnerID=8YFLogxK

U2 - 10.1016/j.ijms.2008.05.032

DO - 10.1016/j.ijms.2008.05.032

M3 - Article

VL - 275

SP - 104

EP - 109

JO - International Journal of Mass Spectrometry

JF - International Journal of Mass Spectrometry

SN - 1387-3806

IS - 1-3

ER -