Tachykinin receptors are involved in the 'local efferent' motor response to capsaicin in the guinea-pig small intestine and oesophagus

L. Barthó, L. Lénárd, R. Patacchini, V. Halmai, M. Wilhelm, P. Holzer, C. A. Maggi

Research output: Contribution to journalArticle

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Abstract

The sensory neuron stimulant drug capsaicin stimulates primary afferent nerve endings in the guinea-pig small intestine, which in turn activate myenteric cholinergic neurons by an unknown mechanism. The tachykinins substance P and neurokinin A are present in primary afferent neurons. This study was performed to assess the possible involvement of endogenous tachykinins acting via neurokinin-1, neurokinin-2 and neurokinin-3 receptors in the contractile effect of capsaicin in the isolated guinea-pig ileum and oesophagus by using the receptor-specific antagonists GR 82334 (3 μM) for neurokinin-1 receptors, MEN 10627 (3 μM; ileum) or MEN 11420 (1 μM; oesophagus) for neurokinin-2 receptors and SR 142801 (0.1 μM) for neurokinin-3 receptors. In the ileum, the peak contraction evoked by capsaicin (2 μM) was not reduced when tachykinin neurokinin-1, neurokinin-2 or neurokinin-3 receptors were blocked separately, whereas an inhibition of neurokinin-3 receptors diminished the area under the curve of the capsaicin response. A combined blockade of neurokinin-1 and neurokinin-3 receptors significantly depressed the effect of capsaicin; the amplitude of the contractile response was 53.3 ± 3.7% of the maximal longitudinal spasm in control preparations, whereas in the presence of GR 82334 plus SR 142801 it reached only 27.6 ± 5% (P

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalNeuroscience
Volume90
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Neurokinin-3 Receptors
Tachykinin Receptors
Capsaicin
Esophagus
Small Intestine
Guinea Pigs
Tachykinins
Ileum
Multiple Endocrine Neoplasia Type 2b
Neurokinin-2 Receptors
Neurokinin A
Multiple Endocrine Neoplasia Type 1
Neurokinin-1 Receptors
Afferent Neurons
Cholinergic Neurons
Nerve Endings
Spasm
Sensory Receptor Cells
Substance P
Area Under Curve

Keywords

  • Capsaicin
  • Myenteric neurons
  • Oesophagus
  • Small intestine
  • Tachykinin receptors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tachykinin receptors are involved in the 'local efferent' motor response to capsaicin in the guinea-pig small intestine and oesophagus. / Barthó, L.; Lénárd, L.; Patacchini, R.; Halmai, V.; Wilhelm, M.; Holzer, P.; Maggi, C. A.

In: Neuroscience, Vol. 90, No. 1, 1999, p. 221-228.

Research output: Contribution to journalArticle

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