Tachykinin NK1 receptor-mediated inhibitory responses in the guinea- pig small intestine

A. Lecci, R. De Giorgio, L. Barthó, C. Sternini, M. Tramontana, R. Corinaldesi, S. Giuliani, C. A. Maggi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We used in vivo, in vitro studies and immunohistochemistry to elucidate the mechanisms activated by tachykinin NK1 receptors in evoking inhibitory motor response in the guinea-pig small intestine. In vivo, the selective NK1 receptor agonist GR 73,632 produced a dose-dependent suppression of the distension-induced duodenal contractions, and a decrease of basal tone. These effects were reduced by pretreatment with the NK1 receptor antagonist SR 140,333. In L-Nω-nitro-L-arginine methylesther hydrochloride-pretreated animals, the suppressant effect of GR 73,632 on duodenal contractions was reduced, whereas the relaxation of the basal tone was unaffected. In vitro, GR 73,632 evoked a biphasic response consisting of a transient, tetrodotoxin- sensitive inhibitory effect followed by tetrodotoxin-resistant contractions. SR 140,333 blocked both inhibitory and excitatory motor responses induced by GR 73,632. NK1 immunoreactivity was localized to myenteric and submucosal neurons and to interstitial cells of Cajal in the deep muscular plexus of the small intestine. NK1 receptor-expressing neurons had Dogiel type I morphology and many of them were β-nicotinamide adenine phosphate dinucleotide-diaphorase-positive, indicating they are inhibitory neurons. In conclusion, in the guinea-pig small intestine, NK1 receptor stimulation evokes a myogenic excitatory motor response and a neurogenic inhibitory motor response that involves, at least in part, a nitrinergic pathway.

Original languageEnglish
Pages (from-to)91-97
Number of pages7
JournalNeuropeptides
Volume33
Issue number1
DOIs
Publication statusPublished - Feb 1999

Fingerprint

Tachykinin Receptors
Tachykinins
Small Intestine
Guinea Pigs
Tetrodotoxin
Neurons
Arginine
Interstitial Cells of Cajal
NADP
Immunohistochemistry
Animals
Cells
In Vitro Techniques
SR 140333

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Neurology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Biochemistry

Cite this

Lecci, A., De Giorgio, R., Barthó, L., Sternini, C., Tramontana, M., Corinaldesi, R., ... Maggi, C. A. (1999). Tachykinin NK1 receptor-mediated inhibitory responses in the guinea- pig small intestine. Neuropeptides, 33(1), 91-97. https://doi.org/10.1054/npep.1999.0019

Tachykinin NK1 receptor-mediated inhibitory responses in the guinea- pig small intestine. / Lecci, A.; De Giorgio, R.; Barthó, L.; Sternini, C.; Tramontana, M.; Corinaldesi, R.; Giuliani, S.; Maggi, C. A.

In: Neuropeptides, Vol. 33, No. 1, 02.1999, p. 91-97.

Research output: Contribution to journalArticle

Lecci, A, De Giorgio, R, Barthó, L, Sternini, C, Tramontana, M, Corinaldesi, R, Giuliani, S & Maggi, CA 1999, 'Tachykinin NK1 receptor-mediated inhibitory responses in the guinea- pig small intestine', Neuropeptides, vol. 33, no. 1, pp. 91-97. https://doi.org/10.1054/npep.1999.0019
Lecci, A. ; De Giorgio, R. ; Barthó, L. ; Sternini, C. ; Tramontana, M. ; Corinaldesi, R. ; Giuliani, S. ; Maggi, C. A. / Tachykinin NK1 receptor-mediated inhibitory responses in the guinea- pig small intestine. In: Neuropeptides. 1999 ; Vol. 33, No. 1. pp. 91-97.
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