T Lymphocytes are Targets for Platelet- and Trophoblast-Derived Microvesicles During Pregnancy

E. Pap, E. Pállinger, A. Falus, A. A. Kiss, A. Kittel, P. Kovács, E. Búzás

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Microvesicles (MVs) can derive from several cell types and their membranes contain cell surface elements. Their role is increasingly recognized in cell-to-cell communication, as they act as both paracrine and remote messengers, occurring in circulating form as well as in plasma. Successful pregnancy requires a series of interactions between the maternal immune system and the implanted fetus, such that the semi-allograft will not be rejected. These interactions occur at the materno-placental interface and/or at a systemic level. In the present study we identified for the first time the in vivo plasma pattern of the MVs of third-trimester, healthy pregnant women, their cellular origin, and their target cells using flow cytometry and confocal laser microscopy. We searched for the cellular target molecules of thrombocyte-derived MVs with the help of neutralizing antibodies. We examined the in vitro effects of MVs on STAT3 phosphorylation of primary lymphocytes and Jurkat cells. We found that both placental trophoblast-derived and maternal thrombocyte-derived MVs bind to circulating peripheral T lymphocytes, but not to B lymphocytes or NK cells. We were able to show that the P-selectin (CD62P)-PSGL-1 (CD162) interaction is one mechanism binding platelet-derived MVs to T cells. We were also able to demonstrate that MV-lymphocyte interactions induce STAT3 phosphorylation in T cells. Our findings indicate that both thrombocyte- and trophoblast-derived MVs may play an important role in the immunomodulation of pregnancy. We suggest that the transfer of different signals via MVs represents a novel form of communication between the placenta and the maternal immune system, and that MVs contribute to the establishment of stable immune tolerance to the semi-allograft fetus.

Original languageEnglish
Pages (from-to)826-832
Number of pages7
JournalPlacenta
Volume29
Issue number9
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Trophoblasts
Blood Platelets
T-Lymphocytes
Pregnancy
Mothers
Confocal Microscopy
Allografts
Immune System
Fetus
Phosphorylation
Lymphocytes
Immune Tolerance
P-Selectin
Jurkat Cells
Immunomodulation
Third Pregnancy Trimester
Neutralizing Antibodies
Cell Communication
Natural Killer Cells
Placenta

Keywords

  • Human pregnancy
  • Immune regulation
  • Platelet-derived microvesicles
  • Trophoblast-derived microvesicles

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

T Lymphocytes are Targets for Platelet- and Trophoblast-Derived Microvesicles During Pregnancy. / Pap, E.; Pállinger, E.; Falus, A.; Kiss, A. A.; Kittel, A.; Kovács, P.; Búzás, E.

In: Placenta, Vol. 29, No. 9, 09.2008, p. 826-832.

Research output: Contribution to journalArticle

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