T-lymphocyte calcium influx characteristics and their modulation by Kv1.3 and IKCa1 channel inhibitors in the neonate

Gergely Toldi, András Treszl, Vince Pongor, Béla Gyarmati, Tivadar Tulassay, Barna Vásárhelyi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cytokine production in activated T lymphocytes of the term neonate is reduced compared with adults. We aimed to characterize the calcium influx kinetics of activated T lymphocytes in the neonate and to test the functionality and expression of Kv1.3 and IKCa1 lymphocyte potassium channels, important regulators of calcium influx. We isolated lymphocytes from the peripheral blood of nine adults and cord blood of nine term neonates. We measured the calcium influx kinetics with flow cytometry in the Th1, Th2, CD4 and CD8 T-lymphocyte subsets activated with PHA. We determined the sensitivity of calcium influx to specific inhibitors of the Kv1.3 and IKCa1 channels. We also measured Kv1.3 channel expression using specific antibody. With the exception of the CD4 subset, calcium influx kinetics was decreased upon activation in neonatal T lymphocytes compared with adults. Neonatal T lymphocytes were found to be less sensitive to the specific inhibition of Kv1.3 and IKCa1 channels. The expression of Kv1.3 channels was higher on major T-lymphocyte subsets of newborns except for Th1 lymphocytes. Our findings suggest that the characteristics of short-term activation of major neonatal T-lymphocyte subsets are altered compared with adults. The altered function of neonatal lymphocyte potassium channels may contribute to this phenomenon.

Original languageEnglish
Pages (from-to)769-774
Number of pages6
JournalInternational Immunology
Volume22
Issue number9
DOIs
Publication statusPublished - Jul 2 2010

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Calcium
T-Lymphocytes
T-Lymphocyte Subsets
Lymphocytes
Potassium Channels
Fetal Blood
Flow Cytometry
Cytokines
Antibodies

Keywords

  • Lymphocyte activation
  • Newborn

ASJC Scopus subject areas

  • Immunology

Cite this

T-lymphocyte calcium influx characteristics and their modulation by Kv1.3 and IKCa1 channel inhibitors in the neonate. / Toldi, Gergely; Treszl, András; Pongor, Vince; Gyarmati, Béla; Tulassay, Tivadar; Vásárhelyi, Barna.

In: International Immunology, Vol. 22, No. 9, 02.07.2010, p. 769-774.

Research output: Contribution to journalArticle

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