T cell receptor (TCR) signal strength controls arthritis severity in proteoglycan-specific TCR transgenic mice

K. Olasz, F. Boldizsar, K. Kis-Toth, O. Tarjanyi, A. Hegyi, W. van Eden, T. A. Rauch, K. Mikecz, T. T. Glant

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

T cell receptor transgenic (TCR-Tg) mice specific for the arthritogenic 5/4E8 epitope in the G1 domain of cartilage proteoglycan were generated and back-crossed into arthritis-prone BALB/c background. Although more than 90% of CD4 + T cells of all TCR-Tg lines were 5/4E8-specific, one (TCR-TgA) was highly sensitive to G1-induced or spontaneous arthritis, while another (TCR-TgB) was less susceptible. Here we studied whether fine differences in TCR signalling controlled the onset and severity of arthritis. Mice from the two TCR-Tg lines were immunized side by side with purified recombinant human G1 (rhG1) domain for G1 domain of cartilage proteoglycan (PG)-induced arthritis (GIA). TCR-TgA mice developed severe and early-onset arthritis, whereas TCR-TgB mice developed weaker arthritis with delayed onset, although TCR-TgB CD4 + T cells expressed approximately twice more TCR-Vβ4 chain protein. The more severe arthritis in TCR-TgA mice was associated with higher amounts of anti-G1 domain-specific antibodies, larger numbers of B cells and activated T helper cells. Importantly, TCR-TgB CD4 + T cells were more sensitive to in vitro activation-induced apoptosis, correlating with their higher TCR and CD3 expression and with the increased TCR signal strength. These findings indicate that TCR signal strength determines the clinical outcome of arthritis induction: 'optimal' TCR signal strength leads to strong T cell activation and severe arthritis in TCR-TgA mice, whereas 'supra-optimal' TCR signal leads to enhanced elimination of self-reactive T cells, resulting in attenuated disease.

Original languageEnglish
Pages (from-to)346-355
Number of pages10
JournalClinical and Experimental Immunology
Volume167
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

    Fingerprint

Keywords

  • Rheumatoid arthritis
  • TcR signalling
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this