T-cell depletion of Cercopithecus aethiops monkey bone marrow with Campath-1 monoclonal antibody and complement.

D. T. Phan, E. Barta, J. Gidáli, I. Fehér, V. Harsányi, G. Petrányi, S. Hollán

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Abstract

The effectiveness of anti-human monoclonal antibody (Campath 1) plus complement (C') in removal of T-cells from Cercopithecus aethiops monkey bone marrow was studied. Recovery of haemopoietic progenitor cells (CFU-GM) was also investigated in vitro after treatment with Campath-1 plus C'. The results showed that the cell-yield was 37.2 +/- 9.8 after Ficoll separation and 44.2 +/- 13.2% after Campath-1 + + C' treatment. The CFU-GM yields referred to the original total CFU-GM were 88.9 +/- 24.0 and 40.0 +/- 14.3%, respectively. After Campath-1 treatment, CFU-GM per 10(5) bone marrow cells was 269.3 as compared to the pretreatment value of 213.0. In the T-cell-depleted bone marrow suspensions mature T lymphocytes could not be detected. Following cryopreservation more than 70% of CFU-GM could be recovered in T-cell depleted bone marrow suspensions kept in frozen state for two months. Cercopithecus aethiops monkeys can be used as a model to study T-cell depletion of bone marrow with Campath-1 plus C' for studying allogeneic bone marrow transplantation.

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalHaematologia
Volume20
Issue number3
Publication statusPublished - 1987

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Cercopithecus aethiops
Granulocyte-Macrophage Progenitor Cells
Haplorhini
Bone Marrow
Monoclonal Antibodies
T-Lymphocytes
Suspensions
Complement C1
Ficoll
Cryopreservation
Homologous Transplantation
Bone Marrow Transplantation
Bone Marrow Cells
Stem Cells
Therapeutics
alemtuzumab

ASJC Scopus subject areas

  • Hematology

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T-cell depletion of Cercopithecus aethiops monkey bone marrow with Campath-1 monoclonal antibody and complement. / Phan, D. T.; Barta, E.; Gidáli, J.; Fehér, I.; Harsányi, V.; Petrányi, G.; Hollán, S.

In: Haematologia, Vol. 20, No. 3, 1987, p. 155-163.

Research output: Contribution to journalArticle

Phan, D. T. ; Barta, E. ; Gidáli, J. ; Fehér, I. ; Harsányi, V. ; Petrányi, G. ; Hollán, S. / T-cell depletion of Cercopithecus aethiops monkey bone marrow with Campath-1 monoclonal antibody and complement. In: Haematologia. 1987 ; Vol. 20, No. 3. pp. 155-163.
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