T-251A polymorphism of IL-8 relating to the development of histological gastritis and G-308A polymorphism of TNF-α relating to the development of macroscopic erosion

Dominika Szoke, Bela Molnar, Norbert Solymosi, Gergely Klausz, Zsofia Gyulai, Bernadett Toth, Yvette Mandi, Zsolt Tulassay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

OBJECTIVES: Genetic variations of the inflammatory IL-8 and TNF-α genes can influence the outcome of gastric alterations. Our aims were to determine the prevalence and effect of the T-251A functional polymorphism of IL-8 and the G-308A polymorphism of TNF-α in histological and macroscopic gastric diseases related to Helicobacter pylori infection. METHODS: Genomic DNA was extracted from biopsy samples from patients with gastritis (n=86, H. pylori positive=41), atrophy (n=32, H. pylori positive=13), intestinal metaplasia (IM) (n=43, H. pylori positive=22) and from histologically negative patients (n=57). The samples were divided by macroscopic diagnosis into erosion and negative groups. The T-251A polymorphism was examined with the amplification refractory mutation system method; the G-308A polymorphism was determined by the polymerase chain reaction-restriction fragment length polymorphism method. For statistical evaluation, Fischer's exact test was used. RESULTS: In the case of T-251A of IL-8, the frequency of the A/A genotype was significantly increased in gastritis (P=0.049) and IM (P=0.038) groups as compared with the histologically negative ones. No relationship was found between macroscopic erosions and H. pylori infection. In the case of G-308A, the G/G genotype frequency was statistically increased in erosions as compared with negative groups (P=0.035). No difference in the distribution of G-308A genotypes in relation to histological alterations and the H. pylori infection was observed. CONCLUSIONS: The effect of the polymorphism of IL-8 seems to be relevant in the pathogenesis of histological gastritis and IM, and the effect of the polymorphism of TNF-α is relevant in the pathogenesis of macroscopic erosive gastritis.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalEuropean Journal of Gastroenterology and Hepatology
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 1 2008

Keywords

  • G-308A polymorphism
  • Gastric diseases
  • Helicobacter pylori
  • IL-8
  • T-251A polymorphism
  • TNF-α

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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