T-251A polymorphism of IL-8 relating to the development of histological gastritis and G-308A polymorphism of TNF-α relating to the development of macroscopic erosion

Dominika Szoke, B. Molnár, N. Solymosi, Gergely Klausz, Zsofia Gyulai, Bernadett Toth, Y. Mándi, Z. Tulassay

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

OBJECTIVES: Genetic variations of the inflammatory IL-8 and TNF-α genes can influence the outcome of gastric alterations. Our aims were to determine the prevalence and effect of the T-251A functional polymorphism of IL-8 and the G-308A polymorphism of TNF-α in histological and macroscopic gastric diseases related to Helicobacter pylori infection. METHODS: Genomic DNA was extracted from biopsy samples from patients with gastritis (n=86, H. pylori positive=41), atrophy (n=32, H. pylori positive=13), intestinal metaplasia (IM) (n=43, H. pylori positive=22) and from histologically negative patients (n=57). The samples were divided by macroscopic diagnosis into erosion and negative groups. The T-251A polymorphism was examined with the amplification refractory mutation system method; the G-308A polymorphism was determined by the polymerase chain reaction-restriction fragment length polymorphism method. For statistical evaluation, Fischer's exact test was used. RESULTS: In the case of T-251A of IL-8, the frequency of the A/A genotype was significantly increased in gastritis (P=0.049) and IM (P=0.038) groups as compared with the histologically negative ones. No relationship was found between macroscopic erosions and H. pylori infection. In the case of G-308A, the G/G genotype frequency was statistically increased in erosions as compared with negative groups (P=0.035). No difference in the distribution of G-308A genotypes in relation to histological alterations and the H. pylori infection was observed. CONCLUSIONS: The effect of the polymorphism of IL-8 seems to be relevant in the pathogenesis of histological gastritis and IM, and the effect of the polymorphism of TNF-α is relevant in the pathogenesis of macroscopic erosive gastritis.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalEuropean Journal of Gastroenterology and Hepatology
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Gastritis
Interleukin-8
Helicobacter pylori
Metaplasia
Helicobacter Infections
Genotype
Stomach Diseases
Restriction Fragment Length Polymorphisms
Atrophy
Stomach
Biopsy
Polymerase Chain Reaction
Mutation
DNA
Genes

Keywords

  • G-308A polymorphism
  • Gastric diseases
  • Helicobacter pylori
  • IL-8
  • T-251A polymorphism
  • TNF-α

ASJC Scopus subject areas

  • Gastroenterology

Cite this

@article{0ee06c0c25084f4da36e9113be266544,
title = "T-251A polymorphism of IL-8 relating to the development of histological gastritis and G-308A polymorphism of TNF-α relating to the development of macroscopic erosion",
abstract = "OBJECTIVES: Genetic variations of the inflammatory IL-8 and TNF-α genes can influence the outcome of gastric alterations. Our aims were to determine the prevalence and effect of the T-251A functional polymorphism of IL-8 and the G-308A polymorphism of TNF-α in histological and macroscopic gastric diseases related to Helicobacter pylori infection. METHODS: Genomic DNA was extracted from biopsy samples from patients with gastritis (n=86, H. pylori positive=41), atrophy (n=32, H. pylori positive=13), intestinal metaplasia (IM) (n=43, H. pylori positive=22) and from histologically negative patients (n=57). The samples were divided by macroscopic diagnosis into erosion and negative groups. The T-251A polymorphism was examined with the amplification refractory mutation system method; the G-308A polymorphism was determined by the polymerase chain reaction-restriction fragment length polymorphism method. For statistical evaluation, Fischer's exact test was used. RESULTS: In the case of T-251A of IL-8, the frequency of the A/A genotype was significantly increased in gastritis (P=0.049) and IM (P=0.038) groups as compared with the histologically negative ones. No relationship was found between macroscopic erosions and H. pylori infection. In the case of G-308A, the G/G genotype frequency was statistically increased in erosions as compared with negative groups (P=0.035). No difference in the distribution of G-308A genotypes in relation to histological alterations and the H. pylori infection was observed. CONCLUSIONS: The effect of the polymorphism of IL-8 seems to be relevant in the pathogenesis of histological gastritis and IM, and the effect of the polymorphism of TNF-α is relevant in the pathogenesis of macroscopic erosive gastritis.",
keywords = "G-308A polymorphism, Gastric diseases, Helicobacter pylori, IL-8, T-251A polymorphism, TNF-α",
author = "Dominika Szoke and B. Moln{\'a}r and N. Solymosi and Gergely Klausz and Zsofia Gyulai and Bernadett Toth and Y. M{\'a}ndi and Z. Tulassay",
year = "2008",
month = "3",
doi = "10.1097/MEG.0b013e3282f1d29f",
language = "English",
volume = "20",
pages = "191--195",
journal = "European Journal of Gastroenterology and Hepatology",
issn = "0954-691X",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - T-251A polymorphism of IL-8 relating to the development of histological gastritis and G-308A polymorphism of TNF-α relating to the development of macroscopic erosion

AU - Szoke, Dominika

AU - Molnár, B.

AU - Solymosi, N.

AU - Klausz, Gergely

AU - Gyulai, Zsofia

AU - Toth, Bernadett

AU - Mándi, Y.

AU - Tulassay, Z.

PY - 2008/3

Y1 - 2008/3

N2 - OBJECTIVES: Genetic variations of the inflammatory IL-8 and TNF-α genes can influence the outcome of gastric alterations. Our aims were to determine the prevalence and effect of the T-251A functional polymorphism of IL-8 and the G-308A polymorphism of TNF-α in histological and macroscopic gastric diseases related to Helicobacter pylori infection. METHODS: Genomic DNA was extracted from biopsy samples from patients with gastritis (n=86, H. pylori positive=41), atrophy (n=32, H. pylori positive=13), intestinal metaplasia (IM) (n=43, H. pylori positive=22) and from histologically negative patients (n=57). The samples were divided by macroscopic diagnosis into erosion and negative groups. The T-251A polymorphism was examined with the amplification refractory mutation system method; the G-308A polymorphism was determined by the polymerase chain reaction-restriction fragment length polymorphism method. For statistical evaluation, Fischer's exact test was used. RESULTS: In the case of T-251A of IL-8, the frequency of the A/A genotype was significantly increased in gastritis (P=0.049) and IM (P=0.038) groups as compared with the histologically negative ones. No relationship was found between macroscopic erosions and H. pylori infection. In the case of G-308A, the G/G genotype frequency was statistically increased in erosions as compared with negative groups (P=0.035). No difference in the distribution of G-308A genotypes in relation to histological alterations and the H. pylori infection was observed. CONCLUSIONS: The effect of the polymorphism of IL-8 seems to be relevant in the pathogenesis of histological gastritis and IM, and the effect of the polymorphism of TNF-α is relevant in the pathogenesis of macroscopic erosive gastritis.

AB - OBJECTIVES: Genetic variations of the inflammatory IL-8 and TNF-α genes can influence the outcome of gastric alterations. Our aims were to determine the prevalence and effect of the T-251A functional polymorphism of IL-8 and the G-308A polymorphism of TNF-α in histological and macroscopic gastric diseases related to Helicobacter pylori infection. METHODS: Genomic DNA was extracted from biopsy samples from patients with gastritis (n=86, H. pylori positive=41), atrophy (n=32, H. pylori positive=13), intestinal metaplasia (IM) (n=43, H. pylori positive=22) and from histologically negative patients (n=57). The samples were divided by macroscopic diagnosis into erosion and negative groups. The T-251A polymorphism was examined with the amplification refractory mutation system method; the G-308A polymorphism was determined by the polymerase chain reaction-restriction fragment length polymorphism method. For statistical evaluation, Fischer's exact test was used. RESULTS: In the case of T-251A of IL-8, the frequency of the A/A genotype was significantly increased in gastritis (P=0.049) and IM (P=0.038) groups as compared with the histologically negative ones. No relationship was found between macroscopic erosions and H. pylori infection. In the case of G-308A, the G/G genotype frequency was statistically increased in erosions as compared with negative groups (P=0.035). No difference in the distribution of G-308A genotypes in relation to histological alterations and the H. pylori infection was observed. CONCLUSIONS: The effect of the polymorphism of IL-8 seems to be relevant in the pathogenesis of histological gastritis and IM, and the effect of the polymorphism of TNF-α is relevant in the pathogenesis of macroscopic erosive gastritis.

KW - G-308A polymorphism

KW - Gastric diseases

KW - Helicobacter pylori

KW - IL-8

KW - T-251A polymorphism

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=39849095056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39849095056&partnerID=8YFLogxK

U2 - 10.1097/MEG.0b013e3282f1d29f

DO - 10.1097/MEG.0b013e3282f1d29f

M3 - Article

C2 - 18301299

AN - SCOPUS:39849095056

VL - 20

SP - 191

EP - 195

JO - European Journal of Gastroenterology and Hepatology

JF - European Journal of Gastroenterology and Hepatology

SN - 0954-691X

IS - 3

ER -