T-2 mycotoxin slows down the development of mouse blastocysts, decreases their Blastomere number and increases chromatin damage

Bence Somoskoi, Melinda Kovács, S. Cseh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The mycotoxin T-2 has many harmful effects on mammalian cells and reproductive functions. In the present study, the in vitro effect of T-2 toxin on mouse blastocysts was examined. Embryos were cultured in media supplemented with 0. 5,0.75 and 1 ng/ml T-2. Different exposure times were applied [96 h (treatment I) or 24 h following 72 h in toxin-free media (treatment II)]. Blastomere number, nuclear chromatin status and blastocoel formation were investigated in blastocysts. Our data show that the effect of T-2 toxin may vary depending on the stage of the embryo at the start of exposure. At 96 h of exposure, the blastocysts had blastomeres with normal chromatin quality but their developmental potential was decreased. After 24 h of exposure applied following a 72-h culture, blastomeres had a higher level of chromatin damage, although their developmental potential was the same as in the control embryos. In both cases, decreased mitotic rate was found, which resulted in decreased blastomere number even at low toxin concentration.

Original languageEnglish
Pages (from-to)390-400
Number of pages11
JournalActa Veterinaria Hungarica
Volume64
Issue number3
DOIs
Publication statusPublished - Sep 1 2016

Keywords

  • Blastocoel
  • Embryotoxicity
  • In vitro culture
  • Mouse embryos
  • Nuclear chromatin
  • T-2 toxin

ASJC Scopus subject areas

  • veterinary(all)

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