Synthetic oligopeptide substrates which fail to compete with h1 histone for type II and type III isoenzymes of protein kinase C

László Buday, Gyöngyi Farkas, Anna Faragó

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Abstract

1. 1. The oligopeptide AAASFKAKK which contains recognition motifs similar to that found in the surrounding of the site of H1 histone phosphorylated by protein kinase C is unable to compete with H1 histone for the type II and type III isoenzymes, though it is a good substrate for protein kinase C and it is able to compete with a physiological substrate of the enzyme. 2. 2. Among several oligopeptides tested as an alternative substrate a very basic peptide proved to be the most effective inhibitor of H1 histone phosphorylation. This oligopeptide substrate contains basic recognition motifs at both sides of the phosphorylated residue at variance with the sequence of H1 histone in the surrounding of the phosphorylated site.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalInternational Journal of Biochemistry
Volume24
Issue number5
DOIs
Publication statusPublished - May 1992

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ASJC Scopus subject areas

  • Biochemistry

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