Synthetic culture media evaluated for detection of coliform bacteria in milk, cheese and egg melange

G. Szita, Bela Gyetvai, J. Szita, M. Gyenes, N. Solymosi, L. Soos, A. Hajos, P. Toth, S. Bernath

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Simple synthetic culture media of liquid and solid form (X broth and X agar) were tested for selective isolation of coliform bacteria. Selectivity is based on the ability of coliform bacteria to grow when the minimal medium contains simple inorganic substances as nitrogen and carbon supply. Selectivity of the media was tested by inoculation of pure cultures of different microbes belonging to the genera of Staphylococcus, Bacillus and Pseudomonas and the family Enterobacteriaceae and was found to be complete in this range. The comparative investigation of milk, camembert cheese and egg melange samples in the traditional and new media proved good applicability of X broth and X agar for an effective and selective detection of coliform bacteria. When testing pasteurized milk samples, X agar detected coliforms in significantly higher counts than violet red-bile-lactose agar.

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalActa Veterinaria Brno
Volume77
Issue number1
DOIs
Publication statusPublished - Mar 2008

Fingerprint

cheese milk
coliform bacteria
Cheese
Agar
Ovum
Culture Media
Milk
culture media
agar
Bacteria
Camembert cheese
pasteurized milk
Bacillus (bacteria)
Enterobacteriaceae
Lactose
bile
Pseudomonas
Staphylococcus
Bile
Bacillus

Keywords

  • Coliform
  • Escherichia coli
  • Food
  • Novel synthetic selective media

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Synthetic culture media evaluated for detection of coliform bacteria in milk, cheese and egg melange. / Szita, G.; Gyetvai, Bela; Szita, J.; Gyenes, M.; Solymosi, N.; Soos, L.; Hajos, A.; Toth, P.; Bernath, S.

In: Acta Veterinaria Brno, Vol. 77, No. 1, 03.2008, p. 143-147.

Research output: Contribution to journalArticle

Szita, G, Gyetvai, B, Szita, J, Gyenes, M, Solymosi, N, Soos, L, Hajos, A, Toth, P & Bernath, S 2008, 'Synthetic culture media evaluated for detection of coliform bacteria in milk, cheese and egg melange', Acta Veterinaria Brno, vol. 77, no. 1, pp. 143-147. https://doi.org/10.2754/avb200877010143
Szita, G. ; Gyetvai, Bela ; Szita, J. ; Gyenes, M. ; Solymosi, N. ; Soos, L. ; Hajos, A. ; Toth, P. ; Bernath, S. / Synthetic culture media evaluated for detection of coliform bacteria in milk, cheese and egg melange. In: Acta Veterinaria Brno. 2008 ; Vol. 77, No. 1. pp. 143-147.
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