Synthesis, solution conformation and interleukin-6-related activities of interleukin-6 peptides

S. Bosze, J. Kajtár, R. Szabó, A. Falus, F. Hudecz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interleukin-6 (IL-6) is a member of the cytokine superfamily characterised by a wide variety of biological activities on various cell types. IL-6 exerts pleiotropic activities on hematopoiesis in the immune response and it is the main regulator of acute-phase protein synthesis in liver cells. According to structure-function studies, residues of helix A located at the N-terminal part and/or helix D of the C-terminal part of the protein are involved in the induction of acute-phase responses. Two groups of synthetic peptides corresponding to the 18-46 N-terminal and the 168-185 C- terminal regions of the IL-6 were prepared by solid-phase synthesis to identify structural requirements for induction of fibrinogen or complement factor B synthesis. These peptides were characterised by amino acid analysis, analytical reversed-phase high-performance liquid chromatography, fast atom bombardment mass spectrometry, and circular dichroism (CD) spectroscopy. CD results showed that under appropriate conditions both 18-46 and 168-185 related peptides are able to adopt markedly ordered conformation. We demonstrated that even octapeptides from the N-terminal part and truncated derivatives of the C-terminal region preserved some tendency to display the CD curve of periodic conformation. The ability of the peptides to induce de novo synthesis of acute-phase proteins was evaluated by measuring fibrinogen and complement factor B levels in the supernatants of human HepG2 cells. These results showed that residues 21-34 are critical for eliciting fibrinogen synthesis in the presence or absence of IL-6. In contrast, the full-length 168-185 peptide is required for the induction of complement factor B response.

Original languageEnglish
Pages (from-to)216-228
Number of pages13
JournalJournal of Peptide Research
Volume52
Issue number3
Publication statusPublished - Sep 29 1998

Keywords

  • Factor B
  • Fibrinogen
  • Interleukin-6
  • Solution conformation
  • Synthetic peptides

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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