Synthesis of tritium-labelled β-amyloid fragments

Éva C. Gulyás, Katalin Soós, József Varga, Géza Tóth, Botond Penke

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2 Citations (Scopus)

Abstract

Two fragments of β-amyloid(1-42) peptide, β-amyloid(31-35) and β- amyloid(25-35), were synthesized and labelled with tritium in the Leu34 residue. Precursor peptides containing ΔLeu34 were prepared by solid phase peptide synthesis using manual Fmoc strategy. Tritium labelling was carried out by catalytic saturation, yielding [3H-Leu34]β-amyloid(31-35) and [3H-Leu34]β-amyloid(25-35) with very high specific activities of around 4.44 TBq/mmol (120 Ci/mmol). The labelled peptides were investigated by RP- HPLC and the distribution of the tritium was determined by acidic hydrolysis followed by RP-HPLC analysis.

Original languageEnglish
Pages (from-to)763-771
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume41
Issue number8
DOIs
Publication statusPublished - Aug 1 1998

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Keywords

  • Catalytic tritiation
  • Solid phase peptides
  • [H- Leu]β-amyloid(31-35),[H-Leu]β-amyloid(25-35)
  • β-amyloid fragments

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Drug Discovery
  • Spectroscopy
  • Organic Chemistry

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