Synthesis of tritium-labelled β-amyloid fragments

E. Hunyadi-Gulyás, K. Soós, József Varga, Géza Tóth, B. Penke

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two fragments of β-amyloid(1-42) peptide, β-amyloid(31-35) and β- amyloid(25-35), were synthesized and labelled with tritium in the Leu34 residue. Precursor peptides containing ΔLeu34 were prepared by solid phase peptide synthesis using manual Fmoc strategy. Tritium labelling was carried out by catalytic saturation, yielding [3H-Leu34]β-amyloid(31-35) and [3H-Leu34]β-amyloid(25-35) with very high specific activities of around 4.44 TBq/mmol (120 Ci/mmol). The labelled peptides were investigated by RP- HPLC and the distribution of the tritium was determined by acidic hydrolysis followed by RP-HPLC analysis.

Original languageEnglish
Pages (from-to)763-771
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume41
Issue number8
DOIs
Publication statusPublished - Aug 1998

Fingerprint

Tritium
Amyloid
Peptides
High Pressure Liquid Chromatography
Solid-Phase Synthesis Techniques
Labeling
Hydrolysis

Keywords

  • β-amyloid fragments
  • [H- Leu]β-amyloid(31-35),[H-Leu]β-amyloid(25-35)
  • Catalytic tritiation
  • Solid phase peptides

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Synthesis of tritium-labelled β-amyloid fragments. / Hunyadi-Gulyás, E.; Soós, K.; Varga, József; Tóth, Géza; Penke, B.

In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 41, No. 8, 08.1998, p. 763-771.

Research output: Contribution to journalArticle

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