Synthesis of 3H-Tyr-D-Ala-Gly-N(Me)Phe chloromethyl ketone-an opioid affinity label

E. Varga, G. Tóth, J. Hepp, S. Benyhe, J. Simon, K. Medzihradszky, A. Borsodi

Research output: Contribution to journalArticle

9 Citations (Scopus)


A radioactive enkephalin affinity reagent, selective for the μ opioid receptor subtype, was synthesized by a fragment condensation method. 3H-BOC-Tyr-D-Ala-Gly-OH was prepared by catalytic tritiation of the protected iodinated tripeptide. The protected tritiated tripeptide and N(Me)Phe-CH2Cl were condensed by the mixed anhydride method. The protecting group was removed by HCl acetic acid. The tritiated tetrapeptide has a specific radioactivity of 56.8 Ci/mmole (2.1 TBq/mmole).

Original languageEnglish
Pages (from-to)135-139
Number of pages5
Issue number3
Publication statusPublished - Oct 1988

ASJC Scopus subject areas

  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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