Two protected peptides Boc‐Val‐Ser(Bzl)‐Gln‐Asn‐Tyr(BrZ)OH and Boc‐Val‐Ser(Bzl)‐Gln‐Asn‐Tyr(BrZ)‐ProOH were synthesized on a resin substituted by 9‐(hydroxymethyl)‐2‐fluoreneacetic acid. After cleavage with piperidine/DMF, desalting, and activation, these peptides were used for the synthesis of 11 analogs of an HIV proteinase nonapeptide substrate Val‐Ser‐Gln‐Asn‐Tyr‐Pro‐Ile‐Val‐Gln‐NH2 using fragment condensation in solid phase. The fragment condensation was made in an ultrasonic bath. Using only 2 equivalents of the activated peptide in a DMF solution, this reaction was complete in 2h. All nonapeptides were assayed as substrates for HIV‐1 and HIV‐2 proteinases.
|Number of pages||6|
|Journal||International journal of peptide and protein research|
|Publication status||Published - Nov 1991|
- HIV proteinase
- fragment condensation
- solid phase peptide synthesis
ASJC Scopus subject areas