Synthesis of chicken galanin and its N- and C-terminal segments, and preparation of their antisera

Lajos Baláspiri, Zsuzsanna Tegyei, Maria Dux, Gábor Jancsó, Rita Józsa, Valér Csernus, Béla Mess

Research output: Contribution to journalArticle

Abstract

We describe the synthesis of the first avian galanin (GAL), chicken GAL, and its N-terminal and C-terminal segments by solid-phase synthesis, using Boc/Bzl amino acid protection groups and MBHA resin. The three peptides were prepared with purities of over 97%, as determined by RP-HPLC, HPCE, FAB-MS or ESI-MS and amino acid analysis. Antibodies against these synthetic peptides were raised in rabbits and used for immunohistochemical localization of GAL- immunoreactive neurons in chicken brain.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalLetters in Peptide Science
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Antibodies
  • Chicken galanin
  • Segments
  • Synthesis

ASJC Scopus subject areas

  • Biochemistry

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