Synthesis of chicken galanin and its N- and C-terminal segments, and preparation of their antisera

Lajos Baláspiri, Zsuzsanna Tegyei, M. Dux, G. Jancsó, R. Józsa, V. Csernus, Béla Mess

Research output: Contribution to journalArticle

Abstract

We describe the synthesis of the first avian galanin (GAL), chicken GAL, and its N-terminal and C-terminal segments by solid-phase synthesis, using Boc/Bzl amino acid protection groups and MBHA resin. The three peptides were prepared with purities of over 97%, as determined by RP-HPLC, HPCE, FAB-MS or ESI-MS and amino acid analysis. Antibodies against these synthetic peptides were raised in rabbits and used for immunohistochemical localization of GAL-immunoreactive neurons in chicken brain.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalInternational Journal of Peptide Research and Therapeutics
Volume7
Issue number1
Publication statusPublished - 2000

Fingerprint

Galanin
Immune Sera
Chickens
Peptides
Amino acids
Amino Acids
Solid-Phase Synthesis Techniques
Antibodies
Neurons
Brain
Resins
High Pressure Liquid Chromatography
Rabbits

Keywords

  • Antibodies
  • Chicken galanin
  • Segments
  • Synthesis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Synthesis of chicken galanin and its N- and C-terminal segments, and preparation of their antisera. / Baláspiri, Lajos; Tegyei, Zsuzsanna; Dux, M.; Jancsó, G.; Józsa, R.; Csernus, V.; Mess, Béla.

In: International Journal of Peptide Research and Therapeutics, Vol. 7, No. 1, 2000, p. 23-26.

Research output: Contribution to journalArticle

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AU - Jancsó, G.

AU - Józsa, R.

AU - Csernus, V.

AU - Mess, Béla

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