Synthesis of β-d-galactopyranoside-presenting glycoclusters, investigation of their interactions with pseudomonas aeruginosa lectin a (Pa-il) and evaluation of their anti-adhesion potential

Lenka Malinovská, Son Thai Le, Mihály Herczeg, Michaela Vašková, Josef Houser, Eva Fujdiarová, Jan Komárek, Petr Hodek, Anikó Borbás, Michaela Wimmerová, Magdolna Csávás

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Abstract

Pseudomonas aeruginosa is an opportunistic human pathogen associated with cystic fibrosis. This bacterium produces, among other virulence factors, a soluble D-galactose-specific lectin PA-IL (LecA). PA-IL plays an important role in the adhesion to the host cells and is also cytotoxic. Therefore, this protein is an interesting therapeutic target, suitable for inhibition by carbohydrate-based compounds. In the current study, β-D-galactopyranoside-containing tri-and tetravalent glycoclusters were synthesized. Methyl gallate and pentaerythritol equipped with propargyl groups were chosen as multivalent scaffolds and the galactoclusters were built from the above-mentioned cores by coupling ethylene or tetraethylene glycol-bridges and peracetylated propargyl β-D-galactosides using 1,3-dipolar azide-alkyne cycloaddition. The interaction between galactoside derivatives and PA-IL was investigated by several biophysical methods, including hemagglutination inhibition assay, isothermal titration calorimetry, analytical ultracentrifugation, and surface plasmon resonance. Their ability to inhibit the adhesion of P. aeruginosa to bronchial cells was determined by ex vivo assay. The newly synthesized multivalent galactoclusters proved to be significantly better ligands than simple D-galactose for lectin PA-IL and as a result, two representatives of the dendrimers were able to decrease adhesion of P. aeruginosa to bronchial cells to approximately 32% and 42%, respectively. The results may provide an opportunity to develop anti-adhesion therapy for the treatment of P. aeruginosa infection.

Original languageEnglish
Article number686
JournalBiomolecules
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 2019

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Keywords

  • Cystic fibrosis
  • D-galactosides
  • Lectin
  • Multivalency
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Malinovská, L., Le, S. T., Herczeg, M., Vašková, M., Houser, J., Fujdiarová, E., Komárek, J., Hodek, P., Borbás, A., Wimmerová, M., & Csávás, M. (2019). Synthesis of β-d-galactopyranoside-presenting glycoclusters, investigation of their interactions with pseudomonas aeruginosa lectin a (Pa-il) and evaluation of their anti-adhesion potential. Biomolecules, 9(11), [686]. https://doi.org/10.3390/biom9110686