Spent Brewing Grains (SBG) was evaluated for its efficacy to be used as sole carbon source for the synthesis of α-amylase in solid-state fermentation using a fungal strain of Aspergillus oryzae NRRL 6270. Enzyme production was superior when the culture grew on mesophilic temperatures and best yields were at 25°C. At 30°C, yields were almost comparable. Maximum production of α-amylase [6870 U/g dry substrate (gds)] was obtained when SSF was carried out at 30°C for 96 h using SBG medium, which had initial moisture of 70% and was inoculated using a spore suspension containing 1 × 107 spores/ml. Supplementation of SBG with external carbon sources such as mono-, di and polysaccharides caused repression in enzyme synthesis by the fungal culture.
|Number of pages||7|
|Journal||Journal of basic microbiology|
|Publication status||Published - Nov 6 2002|
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology