Synthesis of α-amylase by Aspergillus oryzae in solid-state fermentation

Febe Francis, A. Sabu, K. Madhavan Nampoothiri, G. Szakács, Ashok Pandey

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Spent Brewing Grains (SBG) was evaluated for its efficacy to be used as sole carbon source for the synthesis of α-amylase in solid-state fermentation using a fungal strain of Aspergillus oryzae NRRL 6270. Enzyme production was superior when the culture grew on mesophilic temperatures and best yields were at 25°C. At 30°C, yields were almost comparable. Maximum production of α-amylase [6870 U/g dry substrate (gds)] was obtained when SSF was carried out at 30°C for 96 h using SBG medium, which had initial moisture of 70% and was inoculated using a spore suspension containing 1 × 107 spores/ml. Supplementation of SBG with external carbon sources such as mono-, di and polysaccharides caused repression in enzyme synthesis by the fungal culture.

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalJournal of Basic Microbiology
Volume42
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

Aspergillus oryzae
Amylases
Spores
Fermentation
Enzyme Repression
Carbon
Polysaccharides
Suspensions
Temperature
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

Synthesis of α-amylase by Aspergillus oryzae in solid-state fermentation. / Francis, Febe; Sabu, A.; Madhavan Nampoothiri, K.; Szakács, G.; Pandey, Ashok.

In: Journal of Basic Microbiology, Vol. 42, No. 5, 2002, p. 320-326.

Research output: Contribution to journalArticle

Francis, Febe ; Sabu, A. ; Madhavan Nampoothiri, K. ; Szakács, G. ; Pandey, Ashok. / Synthesis of α-amylase by Aspergillus oryzae in solid-state fermentation. In: Journal of Basic Microbiology. 2002 ; Vol. 42, No. 5. pp. 320-326.
@article{4976dc97846a4cab86097b6b879ef1c2,
title = "Synthesis of α-amylase by Aspergillus oryzae in solid-state fermentation",
abstract = "Spent Brewing Grains (SBG) was evaluated for its efficacy to be used as sole carbon source for the synthesis of α-amylase in solid-state fermentation using a fungal strain of Aspergillus oryzae NRRL 6270. Enzyme production was superior when the culture grew on mesophilic temperatures and best yields were at 25°C. At 30°C, yields were almost comparable. Maximum production of α-amylase [6870 U/g dry substrate (gds)] was obtained when SSF was carried out at 30°C for 96 h using SBG medium, which had initial moisture of 70{\%} and was inoculated using a spore suspension containing 1 × 107 spores/ml. Supplementation of SBG with external carbon sources such as mono-, di and polysaccharides caused repression in enzyme synthesis by the fungal culture.",
author = "Febe Francis and A. Sabu and {Madhavan Nampoothiri}, K. and G. Szak{\'a}cs and Ashok Pandey",
year = "2002",
doi = "10.1002/1521-4028(200210)42:5<320::AID-JOBM320>3.0.CO;2-6",
language = "English",
volume = "42",
pages = "320--326",
journal = "Journal of Basic Microbiology",
issn = "0233-111X",
publisher = "Wiley-VCH Verlag",
number = "5",

}

TY - JOUR

T1 - Synthesis of α-amylase by Aspergillus oryzae in solid-state fermentation

AU - Francis, Febe

AU - Sabu, A.

AU - Madhavan Nampoothiri, K.

AU - Szakács, G.

AU - Pandey, Ashok

PY - 2002

Y1 - 2002

N2 - Spent Brewing Grains (SBG) was evaluated for its efficacy to be used as sole carbon source for the synthesis of α-amylase in solid-state fermentation using a fungal strain of Aspergillus oryzae NRRL 6270. Enzyme production was superior when the culture grew on mesophilic temperatures and best yields were at 25°C. At 30°C, yields were almost comparable. Maximum production of α-amylase [6870 U/g dry substrate (gds)] was obtained when SSF was carried out at 30°C for 96 h using SBG medium, which had initial moisture of 70% and was inoculated using a spore suspension containing 1 × 107 spores/ml. Supplementation of SBG with external carbon sources such as mono-, di and polysaccharides caused repression in enzyme synthesis by the fungal culture.

AB - Spent Brewing Grains (SBG) was evaluated for its efficacy to be used as sole carbon source for the synthesis of α-amylase in solid-state fermentation using a fungal strain of Aspergillus oryzae NRRL 6270. Enzyme production was superior when the culture grew on mesophilic temperatures and best yields were at 25°C. At 30°C, yields were almost comparable. Maximum production of α-amylase [6870 U/g dry substrate (gds)] was obtained when SSF was carried out at 30°C for 96 h using SBG medium, which had initial moisture of 70% and was inoculated using a spore suspension containing 1 × 107 spores/ml. Supplementation of SBG with external carbon sources such as mono-, di and polysaccharides caused repression in enzyme synthesis by the fungal culture.

UR - http://www.scopus.com/inward/record.url?scp=0036407213&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036407213&partnerID=8YFLogxK

U2 - 10.1002/1521-4028(200210)42:5<320::AID-JOBM320>3.0.CO;2-6

DO - 10.1002/1521-4028(200210)42:5<320::AID-JOBM320>3.0.CO;2-6

M3 - Article

C2 - 12362403

AN - SCOPUS:0036407213

VL - 42

SP - 320

EP - 326

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

SN - 0233-111X

IS - 5

ER -